ZFIN ID: ZDB-FIG-120320-5
Takesono et al., 2012 - Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo. Proceedings of the National Academy of Sciences of the United States of America   109(9):3371-3376 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 1k-cell to Oblong

Fig. 2

Loss of function of Slc3a2 enhances cell–cell fusion and YSN clustering. (A) Sequences of TPF time-lapse images showing GFP membrane (Upper) and outlines of marginal cells and the YSL (Lower). Fusing cells are indicated by colored outlines, and fusion locations are indicated by colored arrows. The width of the YSL at the outer edge is shown by a white bracket. Cell–cell fusion occurs mostly around the 512-cell stage (red arrow in control, red and green arrows in MOs) but persists through the following stages in slc3a2-MOs (MOs) (pink and cyan arrows in MOs). (B) Merged images of CARS (for lipid; blue) and TPF (for GFP membrane; green) of control and MOs. Uniformly distributed YSN in the control are indicated by white arrowheads, and clustered YSN in MOs are indicated by red asterisks. Multinucleated EVL cells are indicated by red brackets.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions 1k-cell - Oblong external yolk syncytial layer increased size, abnormal
1k-cell - Oblong syncytium formation by plasma membrane fusion increased occurrence, abnormal
1k-cell - Oblong yolk syncytial layer nucleus aggregated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA