ZFIN ID: ZDB-FIG-120320-7
Takesono et al., 2012 - Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo. Proceedings of the National Academy of Sciences of the United States of America   109(9):3371-3376 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Dome

Fig. 4

Slc3a2 inhibits the RhoA/ROCK pathway via c-Src activation in regulating YSL organization. (A) slc3a2 knockdown reduces tyrosine phosphorylation of c-Src. Western blot shows pY416 c-Src and total c-Src in lysates from control, slc3a2-morphants (MOs), or slc3a2/RhoA morphants (MOs+RhoA-MO). Data shown are representative of three independent experiments. (B) Overexpression of v-Src restores YSN localization after slc3a2 knockdown. The YSN are visualized by Sytox green. Clustered YSN in MOs (white arrows) are restored by coinjecting v-Src mRNA (v-Src+MOs). (C) Overexpression of v-Src restores microtubule networks in the YSL in slc3a2-knockdown embryos. YSL microtubule networks (╬▒TUB; red), YSN distribution (SytG; green), and overlap of YSN and YSL microtubules (white squares in merged images) are all restored by v-Src+MOs.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions Dome regulation of Rho-dependent protein serine/threonine kinase activity process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA