ZFIN ID: ZDB-FIG-120320-9
Takesono et al., 2012 - Solute carrier family 3 member 2 (Slc3a2) controls yolk syncytial layer (YSL) formation by regulating microtubule networks in the zebrafish embryo. Proceedings of the National Academy of Sciences of the United States of America   109(9):3371-3376 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. S2

Loss of function of Slc3a2 induces morphological defects but does not affect patterning. (A) Morphological phenotypes caused by slc3a2 knockdown. (a) Control embryo. (b-e) Morphant embryo injected with slc3a2-b-MO1 (b); slc3a2-a-MO1 (c); a combination of slc3a2-a-MO1 and slc3a2-b-MO1 (slc3a2-MOs1) (d); or a combination of slc3a2-a-MO2 and slc3a2-b-MO2 (slc3a2-MOs2) (e). (f) Embryo overexpressing HN-slc3a2-b (HNslc3a2b) (Material and Methods). Single gene knockdown by morpholino causes mild phenotypes (b and c), whereas double knockdown of both slc3a2-a and slc3a2–b by a set of MO1 or MO2 (d and e) or overexpression of HN-slc3a2-b (f) induces severe morphological defects with a smaller head and shorter tail. Images are 1-day-old embryos. (B) Phenotypes at 24 h postfertilization were categorized as normal (white), mild (light gray), severe (small head and short body axis) (dark gray), or dead (black). Representatives of mild and severe phenotypes in morphants are shown in Fig. 1B. Data shown are from three experiments, and the total number of embryos is shown below each bar. (C) Whole-mount in situ analyses for MyoD (a and b) and pax2 (c and d) in control and slc3a2-morphants (MOs). Note that expression patterns of MyoD and pax2 are retained despite severe morphological changes.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 WT control Prim-5 myotome ISH
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions Prim-5 myotome ISH
pax2a WT control Prim-5 midbrain hindbrain boundary ISH
Prim-5 optic stalk ISH
Prim-5 otic vesicle ISH
Prim-5 pronephric duct ISH
Prim-5 spinal cord ISH
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions Prim-5 midbrain hindbrain boundary ISH
Prim-5 optic stalk ISH
Prim-5 otic vesicle ISH
Prim-5 pronephric duct ISH
Prim-5 spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-slc3a2a standard conditions Prim-5 post-vent region curved ventral, abnormal
WT + MO1-slc3a2a + MO1-slc3a2b standard conditions Prim-5 head decreased size, abnormal
Prim-5 post-vent region decreased length, abnormal
WT + MO1-slc3a2a + MO1-slc3a2b + MO2-slc3a2a + MO2-slc3a2b standard conditions Prim-5 head decreased size, abnormal
Prim-5 post-vent region decreased length, abnormal
WT + MO1-slc3a2b standard conditions Prim-5 head size, normal
Prim-5 post-vent region length, normal
WT + MO2-slc3a2a + MO2-slc3a2b standard conditions Prim-5 head decreased size, abnormal
Prim-5 post-vent region decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA