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ZIRC
ZFIN ID: ZDB-FIG-111128-65
Zuniga et al., 2011 - Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton. Development (Cambridge, England)   138(23):5147-5156 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Prim-25
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 7 Grem2 promotes the dorsal and intermediate facial skeleton. (A) Structure of the grem2 gene and the Grem2:GFP fusion construct. grem2-MO #1 recognizes the ATG start site in Exon 2 and grem2-MO #2 recognizes the 5′ UTR. (B,C) Grem2:GFP fluorescence in uninjected (B) or grem2-MO-#1-injected (C) embryos at 9 hpf. (D,E) Confocal sections of 30 hpf BRE:GFP transgenic embryos. Compared with uninjected controls (D, n=5), injection of grem2-MO-#1 (E, n=8) increased BRE:GFP throughout the mandibular (1) and hyoid (2) arches. (F-H) Confocal sections of in situ hybridizations for hand2 (green) and dlx3b (red). Relative to the first pouch (white outlines), dlx3b and hand2 were mildly expanded in grem2-MO embryos (G, n=45/52) and lost in hsp70I:Gal4; UAS:Grem2 embryos subjected to a 16-17 hpf heat-shock (H, n=16/16). Arrow indicates expanded hand2 expression. (I-M) Ventral views of 5 dpf facial skeletons from control (I), grem2-MO-#1-injected (J), UAS:Grem2 (K), edn1–/– mutant (L) and an edn1–/– mutant injected with grem2-MO-#1 (M). Schematics show skeletal regions derived from dorsal (green), intermediate (red) and ventral (blue) arch domains, with bones lightly shaded. Elements of undefined morphology or derived from the maxillary domain or more posterior arches are grey. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b el326Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
kca4Tg + MO1-grem2b standard conditions Prim-25 pharyngeal arch 1 ISH
GFP ir1189Tg standard conditions Prim-15 pharyngeal arch 1 IFL
Prim-15 pharyngeal arch 2 IFL
ir1189Tg + MO1-grem2b standard conditions Prim-15 pharyngeal arch 1 IFL
Prim-15 pharyngeal arch 2 IFL
hand2 el326Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch cranial neural crest cell ISH
kca4Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
kca4Tg + MO1-grem2b standard conditions Prim-25 pharyngeal arch cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-grem2b standard conditions Day 5 hyomandibula absent, abnormal
Day 5 opercle malformed, abnormal
Day 5 pharyngeal arch 1 malformed, abnormal
Day 5 pharyngeal arch 2 malformed, abnormal
edn1tf216b/tf216b standard conditions Day 5 pharyngeal arch 1 malformed, abnormal
Day 5 pharyngeal arch 2 malformed, abnormal
edn1tf216b/tf216b; el326Tg; kca4Tg heat shock Day 5 pharyngeal arch 1 malformed, abnormal
Day 5 pharyngeal arch 2 malformed, abnormal
el326Tg; kca4Tg heat shock Day 5 pharyngeal arch 1 malformed, abnormal
Day 5 pharyngeal arch 2 malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development