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ZFIN ID: ZDB-FIG-111128-63
Zuniga et al., 2011 - Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton. Development (Cambridge, England)   138(23):5147-5156 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Prim-25

Fig. 5 Bmp4 induces hand2 and msxe expression in the absence of Edn1. (A-P) Confocal sections of 36 hpf in situ hybridizations for hand2 (green) with dlx3b (red, A-H) or msxe (red, I-P) in control hsp70I:Gal4 (A,I), edn1-/- mutant (E,M), UAS:Bmp4 (B-D,J-L) and edn1-/-; UAS:Bmp4 (F-H,N-P) embryos. Increasing periods of Bmp4 heat-shock induction [1 minute at 21 hpf (B,F,J,N), 3 minutes at 21 hpf (C,G,K,O) and 4 hours from 20-24 hpf (D,H,L,P)] resulted in progressive recovery of hand2 and msxe but not dlx3b expression in edn1-/- mutants. Consistent phenotypes were observed for the following: (A) n=55, (B) n=31, (C) n=15, (D) n=9, (E) n=8, (F) n=3, (G) n=4, (H) n=1, (I) n=37, (J) n=11, (K) n=6, (L) n=9, (M) n=20, (N) n=2, (O) n=3 and (P) n=2. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b edn1tf216b/tf216b heat shock Prim-25 Not Detected pharyngeal arch 1 central region ISH
edn1tf216b/tf216b; el49Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch 1 central region ISH
el49Tg; kca4Tg heat shock Prim-25 mandibular arch skeleton ISH
kca4Tg heat shock Prim-25 pharyngeal arch 1 central region ISH
hand2 edn1tf216b/tf216b heat shock Prim-25 Not Detected pharyngeal arch cranial neural crest cell ISH
edn1tf216b/tf216b; el49Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch cranial neural crest cell ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
kca4Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
msx1a edn1tf216b/tf216b heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
edn1tf216b/tf216b; el49Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch 1 ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
kca4Tg heat shock Prim-25 pharyngeal arch 1 ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development