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ZFIN ID: ZDB-FIG-111128-64
Zuniga et al., 2011 - Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton. Development (Cambridge, England)   138(23):5147-5156 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Prim-25

Fig. 6 Edn1 and Jag1b negatively regulate grem2 expression. (A-F) Confocal sections of in situ hybridization for grem2 (red) and dlx2a (green) in controls (A, n=62), jag1bb1105 mutant (B, n=9), edn1-/- mutant (C, n=5), UAS:Edn1 (D, n=17), hsp70I:dnBmpr1a-GFP (E, n=32) and UAS:Bmp4 (F, n=23) embryos at 36 hpf. In UAS:Edn1 embryos, grem2 expression was seen throughout the mandibular and hyoid arches, except for the ventral-most domain (white arrow). In hsp70I:dnBmpr1a-GFP embryos, grem2 expression shifted to ventral regions (yellow arrow) and was reduced dorsally (white arrowhead). Dorsal (D), intermediate (I) and ventral (V) domains of the mandibular (1) and hyoid (2) arches are labeled. Scale bar: 50 μm. (G,H) BRE:GFP expression increased throughout the dorsal and intermediate arch domains of edn1-/- mutants (n=4) compared with wild-type siblings (n=16).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT control Prim-25 pharyngeal arch cranial neural crest cell ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch cranial neural crest cell ISH
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
el49Tg; kca4Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch cranial neural crest cell ISH
w30Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
GFP edn1tf216b/tf216b; ir1189Tg standard conditions Prim-25 pharyngeal arch cranial neural crest cell IFL
ir1189Tg standard conditions Prim-25 pharyngeal arch cranial neural crest cell IFL
grem2b WT control Prim-25 pharyngeal arch cranial neural crest cell ISH
edn1tf216b/tf216b standard conditions Prim-25 pharyngeal arch cranial neural crest cell ISH
el249Tg; kca4Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
el49Tg; kca4Tg heat shock Prim-25 Not Detected pharyngeal arch cranial neural crest cell ISH
jag1bb1105/b1105 standard conditions Prim-25 pharyngeal arch cranial neural crest cell ISH
w30Tg heat shock Prim-25 pharyngeal arch cranial neural crest cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development