ZFIN ID: ZDB-FIG-110920-48
McGraw et al., 2011 - Lef1 is required for progenitor cell identity in the zebrafish lateral line primordium. Development (Cambridge, England)   138(18):3921-3930 Full text @ Development
Anatomical Terms:
Stage: Prim-15
Knockdown Reagents:
Observed In:
Stage: Day 6

Fig. S5

Expression of tcf genes in the primordium. At 30 hpf, tcf7 is expressed in the leading zone of the primordium (A), tcf7l1a is expressed throughout the primordium, but not in leading edge cells (B) and tcf7l1b is expressed in a deposited NM, but not in the primordium (C) in a wild-type embryo. Scale bar: 20 ┬Ám. (D) Pectoral fin development in embryos injected with the tcf7-MO in a heterozygous (E) or lef1nl2 mutant (F) background at 6 dpf. Pectoral fins are stunted or absent in the lef1nl2;tcf7-MO embryo when compared with the lef1nl wild-type sibling injected with tcf7-MO (red arrows). Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tcf7 WIK/AB standard conditions Prim-15 posterior lateral line primordium ISH
tcf7l1a WIK/AB standard conditions Prim-15 posterior lateral line primordium ISH
tcf7l1b WIK/AB standard conditions Prim-15 neuromast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lef1nl2/nl2 + MO1-tcf7 + MO4-tp53 standard conditions Day 6 pectoral fin hypoplastic, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development