ZFIN ID: ZDB-FIG-110920-44
McGraw et al., 2011 - Lef1 is required for progenitor cell identity in the zebrafish lateral line primordium. Development (Cambridge, England)   138(18):3921-3930 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. S1

lef1nl2 mutants lack terminal NMs and have a truncated pLL nerve. (A,B) Wild-type sibling and lef1nl2 mutant larvae at 4 dpf. The pLL nerve is visualized by expression of the TgBAC(neurod:EGFP) transgene. NMs are labeled with DASPEI. (A) Wild-type embryo contains NMs L1-L5, a secondary (2°) NM and the terminal cluster (tc). (B) lef1nl2 mutant contains NMs L1-L5 and a 2° NM, but lacks a terminal cluster (tc). In addition, the lef1nl2 mutant has a truncated pLL nerve (white arrow). (C,D) Functional hair cells in the final deposited NM labeled with FM 1-43 (red) in wild-type (C) and lef1nl2 larvae (D) at 4 dpf. Nuclei are labeled with DAPI. (E) Quantification of FM 1-43-labeled hair cells showing no significant difference between wild-type and mutant larvae (F) Average number of DAPI-labeled nuclei per NM in wild-type and lef1nl2 (n=6, mean ± s.e.m.). Scale bar: 20 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP lef1nl2/nl2; nl1Tg standard conditions Day 4 posterior lateral line nerve IFL
nl1Tg standard conditions Day 4 posterior lateral line nerve IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lef1nl2/nl2; nl1Tg standard conditions Day 4 posterior lateral line nerve truncated, abnormal
Day 4 posterior lateral line neuromast posterior-most region absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development