ZFIN ID: ZDB-FIG-110920-28
McGraw et al., 2011 - Lef1 is required for progenitor cell identity in the zebrafish lateral line primordium. Development (Cambridge, England)   138(18):3921-3930 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 1-cell to Adult
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 2

The lef1nl2 mutant contains a lesion in the lef1 gene. (A) The lef1nl2 mutation was mapped to a 460 Kb region on chromosome 1. Numbers of recombinants are indicated under each marker. (B) The lef1nl2 mutation is a single guanine insertion at position 1120 (red asterisk). (C) The resulting frame shift is predicted to disrupt the nuclear localization signal (NLS) and extend the protein by 29 amino acids (aa; also shown a β-catenin binding domain and HMG Box). (D) Western blot of wild-type, lef1nl2 mutant and lef1 morphant whole embryo lysates probed with anti-Lef1 antibody and anti-α actin antibody. (E-F′) Immunolabeling using anti-Lef1 antibody revealed nuclear labeling (red) in wild-type sibling and cytoplasmic labeling in lef1nl2 mutant embryos. Nuclei are labeled with DAPI. (G-I) Wild-type sibling, lef1nl2 mutant and lef1-MO injected embryos expressing the Tg(–8.0cldnb:lynGFP) transgene at 2 dpf. (G) The pLL was truncated prematurely (white arrows) in the lef1nl2 mutant (H) and lef1 morphant (I). (J) The axial positions of the deposited NMs are shifted anteriorally in lef1nl2 mutants when compared with wild-type siblings and in lef1 morphants when compared with uninjected controls. (Data presented as mean±s.d.; n=6-11 P<0.001, two-way ANOVA with replication.) Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP lef1nl2/nl2; zf106Tg standard conditions Long-pec posterior lateral line IFL
zf106Tg standard conditions Long-pec posterior lateral line IFL
zf106Tg + MO4-lef1 standard conditions Long-pec posterior lateral line IFL
lef1 Ab1-lef1 WIK/AB standard conditions 1-cell to Adult whole organism WB
Ab1-lef1 Prim-15 posterior lateral line primordium nucleus IHC
Ab1-lef1 WIK/AB + MO4-lef1 standard conditions 1-cell to Adult Not Detected whole organism WB
Ab1-lef1 lef1nl2/nl2(WIK/AB) standard conditions 1-cell to Adult whole organism WB
Ab1-lef1 Prim-15 posterior lateral line primordium cytoplasm IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lef1nl2/nl2; zf106Tg standard conditions Long-pec posterior lateral line neuromast mislocalised anteriorly, abnormal
Long-pec posterior lateral line truncated, abnormal
zf106Tg + MO4-lef1 standard conditions Long-pec posterior lateral line neuromast mislocalised anteriorly, abnormal
Long-pec posterior lateral line truncated, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development