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ZIRC
ZFIN ID: ZDB-FIG-110526-14
Knight et al., 2011 - Ret signalling integrates a craniofacial muscle module during development. Development (Cambridge, England)   138(10):2015-2024 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 3 stmhy024 mutants show myogenic defects specifically in opercular muscles. (A-H) In situ hybridisation with tbx1 (A,B) and capsulin (C,D) probes reveals that muscle specification is unaffected in the pharyngeal arch muscle precursors of stmhy024. However, both myf5 (E,F) and myod (G,H) expression are reduced in stmhy024 relative to wild type (WT). Arrowheads indicate muscle primordia. (I-L) Fluorescent in situ reveals that myod expression is reduced specifically in the precursors of the lo and ao at 52 hours post-fertilisation (hpf; I,J) but is unaffected in the adjacent ah. At later stages, there are fewer En2-expressing (En2+) muscle cells specifically in the do but not the lap of stmhy024 (K,L). Brackets indicate do muscle cell nuclei. (M) Quantification of En2+ muscle precursors in stmhy024 (blue) and WT (red) at 48, 52 and 56 hpf reveals that stmhy024 mutants have fewer muscle precursor cells than WT in the lap or do primordia from 56 hpf (mean ± s.e.m.). Pharyngeal arches 1-7 are shown by numbering in A-F. ah, adductor hyoideous; ao, adductor operculi; do, dilator operculi; e, eye; lap, levator arcus palatini; lo, levator operculi. Scale bars: 50 μm in A-H,K-L; 20 μm in I,J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myf5 WT standard conditions Prim-15 pharyngeal arch myoblast ISH
gfra3hh024/hh024 standard conditions Prim-15 pharyngeal arch myoblast ISH
myod1 WT standard conditions Long-pec dilatator operculi myoblast ISH
Long-pec hyoid muscle myoblast ISH
Long-pec levator arcus palatini myoblast ISH
gfra3hh024/hh024 standard conditions Long-pec dilatator operculi myoblast ISH
Long-pec hyoid muscle myoblast ISH
Long-pec levator arcus palatini myoblast ISH
tbx1 WT standard conditions Prim-15 pharyngeal arch myoblast ISH
gfra3hh024/hh024 standard conditions Prim-15 pharyngeal arch myoblast ISH
tcf21 WT standard conditions High-pec pharyngeal arch myoblast ISH
gfra3hh024/hh024 standard conditions High-pec pharyngeal arch myoblast ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC WT standard conditions Protruding-mouth dilatator operculi myoblast
IHC Protruding-mouth levator arcus palatini myoblast
IHC Protruding-mouth pharyngeal arch 1 myoblast
IHC gfra3hh024/hh024 standard conditions Protruding-mouth dilatator operculi myoblast
IHC Protruding-mouth levator arcus palatini myoblast
IHC Protruding-mouth pharyngeal arch 1 myoblast
Phenotype Details
Fish Conditions Stage Phenotype
gfra3hh024/hh024 standard conditions Long-pec adductor hyomandibulae myoblast amount, normal
Long-pec adductor operculi myoblast decreased amount, abnormal
Long-pec branchiomeric skeletal muscle development process quality, abnormal
Long-pec levator operculi myoblast decreased amount, abnormal
Protruding-mouth branchiomeric skeletal muscle development process quality, abnormal
Protruding-mouth dilatator operculi myoblast decreased amount, abnormal
Protruding-mouth levator arcus palatini myoblast amount, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development