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ZFIN ID: ZDB-FIG-110526-13
Knight et al., 2011 - Ret signalling integrates a craniofacial muscle module during development. Development (Cambridge, England)   138(10):2015-2024 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1 Musculoskeletal labelling of stmhy024 zebrafish mutants reveals specific muscle defects. (A-D) Immunolabelling of muscle (red) and cartilage (green) reveals that stmhy024 mutants (B,D) have reduced dilator operculi (do), adductor operculi (ao), levator operculi (lo) and hyohyoideus superiores (hh sup) muscles (arrowheads) relative to wild type (WT; A,C). e, eye. (E,F) stmhy024 mutants (F) have tightly closed mouths (arrow) relative to WT (E). (G,H) Higher magnification views of dorsal pharyngeal arch muscles reveal that the do, ao and lo attaching to the opercular bone (op, green) are reduced in stmhy024; the non-opercular muscles levator arcus palatini (lap) and adductor hyoideous (ah) are unaffected. (I) Quantification of fibre number in dorsal arch muscles (mean ± s.e.m.) reveals a specific and significant reduction (*P<0.0001) of the opercular muscles in stmhy024 relative to WT (n=10), during muscle development at 72, 96 and 120 hours post-fertilisation (hpf). Scale bars: 100 μm in A-D; 50 μm in G,H.

Gene Expression DetailsNo data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-MF20 IHC WT standard conditions Day 5 abductor hyohyoid
IHC Day 5 adductor hyohyoid
IHC Day 5 adductor hyomandibulae
IHC Day 5 adductor operculi
IHC Day 5 dilatator operculi
IHC Day 5 inferior hyohyoid
IHC Day 5 levator arcus palatini
IHC Day 5 levator operculi
IHC gfra3hh024/hh024 standard conditions Day 5 adductor hyomandibulae
IHC Day 5 adductor operculi
IHC Day 5 dilatator operculi
IHC Day 5 inferior hyohyoid
IHC Day 5 levator arcus palatini
IHC Day 5 levator operculi
Ab1-col2a IHC WT standard conditions Day 5 cranial cartilage
IHC Day 5 opercle
IHC gfra3hh024/hh024 standard conditions Day 5 Not Detected abductor hyohyoid
IHC Day 5 Not Detected adductor hyohyoid
IHC Day 5 cranial cartilage
IHC Day 5 opercle
Phenotype Details
Fish Conditions Stage Phenotype
gfra3hh024/hh024 standard conditions Day 5 abductor hyohyoid lacks parts or has fewer parts of type skeletal muscle cell, abnormal
Day 5 adductor hyohyoid lacks parts or has fewer parts of type skeletal muscle cell, abnormal
Day 5 adductor operculi has fewer parts of type skeletal muscle cell, abnormal
Day 5 branchiomeric skeletal muscle development process quality, abnormal
Day 5 dilatator operculi has fewer parts of type skeletal muscle cell, abnormal
Day 5 levator operculi has fewer parts of type skeletal muscle cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development