ZFIN ID: ZDB-FIG-091217-93
Lee et al., 2009 - FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis. Developmental Biology   336(2):232-245 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: 5-9 somites

Fig. 3 Loss of FoxD5 function did not affect the wavefront and clock in the PSM, but did cause defects in the somites and anterior PSM where the somites were formed. By WISH, the expressions of deltaC, her1, notch2, notch3, ephB2a, paraxis and tbx24, as well as FoxD5 in wild-type embryos (WT), FoxD5 morphants (FoxD5 MO) and tbx24 morphants (tbx24 MO), were observed at 10–14 hpf as indicated. Compared to WT embryos (A, C), FoxD5 morphants showed normal variation in the pattern of deltaC expression, but the striped expression was not maintained in the region where the somites (arrowhead) should have been presented (B, D). The expression of her1 showed a normal oscillation in WT (E) and FoxD5 morphants (F). The striped patterns of notch2, notch3, ephB2a and paraxis in the somites were normal in WT embryos (G, I, K, M), but they were disordered in FoxD5 morphants (H, J, L, N). Expression of papc appeared strongly at somites from S-II to S0. Although the intensity was decreased from posterior to anterior in WT embryos (O), the expression of papc was ectopically increased and showed 5–6 strong signal bands in FoxD5 morphants (P).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT standard conditions Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
WT + MO1-foxd5 standard conditions Bud segmental plate ISH
10-13 somites segmental plate ISH
10-13 somites somite ISH
ephb2a WT standard conditions 5-9 somites somite ISH
WT + MO1-foxd5 standard conditions 5-9 somites somite ISH
her1 WT standard conditions 5-9 somites segmental plate ISH
5-9 somites somite ISH
WT + MO1-foxd5 standard conditions 5-9 somites segmental plate ISH
5-9 somites somite ISH
notch2 WT standard conditions 5-9 somites somite ISH
WT + MO1-foxd5 standard conditions 5-9 somites somite ISH
notch3 WT standard conditions 5-9 somites somite ISH
WT + MO1-foxd5 standard conditions 5-9 somites somite ISH
pcdh8 WT standard conditions 5-9 somites segmental plate ISH
5-9 somites somite ISH
WT + MO1-foxd5 standard conditions 5-9 somites segmental plate ISH
5-9 somites somite ISH
tcf15 WT standard conditions 5-9 somites somite ISH
WT + MO1-foxd5 standard conditions 5-9 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxd5 standard conditions 5-9 somites somite anterior-posterior polarity, abnormal
WT + MO1-foxd5 standard conditions 5-9 somites somite disorganized, abnormal
WT + MO1-foxd5 standard conditions 5-9 somites somitogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 336(2), Lee, H.C., Tseng, W.A., Lo, F.Y., Liu, T.M., and Tsai, H.J., FoxD5 mediates anterior-posterior polarity through upstream modulator Fgf signaling during zebrafish somitogenesis, 232-245, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.