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Radar signaling affects known determinants of dorsal-ventral retinal patterning. Expression patterns are visualized by whole-mount in situ hybridization of eyes from 26-somite embryos. Retinal markers in WT (A-G), radars327 mutants (H-N), and WT overexpressing hsp70:radarWT (O-U). In radars327 mutants, retinal bmp2b expression remained normal, bmp4 expression was severely reduced, tbx2b, tbx5, and efnb2a expression was absent, and vax2 expression was expanded dorsally. In radar-overexpressing embryos, bmp2b, bmp4, tbx2b, tbx5 and efnb2a expression were expanded ventrally, and vax2 was lost. ephA4b was unaffected by either loss or gain of radar function. Eyes overexpressing radar were often small and failed to close at the optic fissure. Neural retina is outlined with dashed line; green bracket identifies optic fissure location and size; red bracket indicates location of ectopic fissures in mutants. (Scale bars, 50 μm.)
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