ZFIN ID: ZDB-FIG-090804-45
Drerup et al., 2009 - Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation. Development (Cambridge, England)   136(15):2623-2632 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 10-13 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 10-13 somites

Fig. 3 Disc1 morphants had a medial expansion of foxd3 and sox10 expression. (A-H) At 10s and 12s, lateral (A-D) and dorsal (E-H) views show the medial expansion and increased level of foxd3 expression in Disc1 morphants (arrows). (I-P) sox10 expression showed similar results. (Q) foxd3 in situ hybridization and Alcian Blue staining of craniofacial cartilage was done on Disc1 morphants and mismatch control-injected embryos from within the same clutch. The ratio of embryos with normal foxd3 expression was significantly reduced in embryos injected with either MOE3 or MOE8. The ratio of larvae from the same clutches with normal Alcian Blue staining (class 1) was similarly reduced. Injection of a mismatch control produced no defect in foxd3 expression or cartilage development. (R) The medial expansion of foxd3 expression can be rescued in Disc1 morphants by co-injection of 100 pg of human DISC1 mRNA (*, P<0.01).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxd3 WT control 10-13 somites cranial neural crest ISH
WT + MO3-disc1 standard conditions 10-13 somites cranial neural crest ISH
WT + MO4-disc1 standard conditions 10-13 somites cranial neural crest ISH
sox10 WT control 10-13 somites cranial neural crest ISH
WT + MO3-disc1 standard conditions 10-13 somites cranial neural crest ISH
WT + MO4-disc1 standard conditions 10-13 somites cranial neural crest ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-disc1 standard conditions 10-13 somites cranial neural crest increased size, abnormal
10-13 somites neural crest cell migration disrupted, abnormal
WT + MO4-disc1 standard conditions 10-13 somites cranial neural crest increased size, abnormal
10-13 somites neural crest cell migration disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development