ZFIN ID: ZDB-FIG-090804-49
Drerup et al., 2009 - Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation. Development (Cambridge, England)   136(15):2623-2632 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 8 Peripheral cranial glia populations were expanded in Disc1 morphants. (A-F) foxd3 expression, which marks the developing glia, was expanded at 48 hpf and 51 hpf, but not 40 hpf, in Disc1 morphants (arrowheads indicate enhanced expression in the trigeminal ganglion and the posterior lateral line ganglion). (G,H) Glial expansion was suppressed by SMO2 co-injection. (I-K) Glial expansion was also suppressed in 49% of Disc1 morphants from a sox10m241/+ by wild-type cross. Uninjected embryos (I; n=47) showed normal foxd3 expression, whereas Disc1 morphants (n=144) showed two distinct phenotypes, expanded (J, arrowheads) and normal (K) foxd3 expression, which probably correlates to their genotype (wild-type or heterozygous). (L) Table showing the percentage of Disc1 morphants with glial expansion (left) and suppression of expansion by SMO2 co-injection (right).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxd3 WT standard conditions Prim-25 posterior lateral line ganglion glial cell ISH
Prim-25 trigeminal ganglion glial cell ISH
Long-pec posterior lateral line ganglion glial cell ISH
Long-pec trigeminal ganglion glial cell ISH
WT + MO3-disc1 standard conditions Prim-25 posterior lateral line ganglion glial cell ISH
Prim-25 trigeminal ganglion glial cell ISH
Long-pec posterior lateral line ganglion glial cell ISH
Long-pec trigeminal ganglion glial cell ISH
WT + MO3-disc1 + MO3-sox10 standard conditions Long-pec posterior lateral line ganglion glial cell ISH
Long-pec trigeminal ganglion glial cell ISH
WT + MO3-sox10 standard conditions Long-pec posterior lateral line ganglion glial cell ISH
Long-pec trigeminal ganglion glial cell ISH
sox10m241/+ standard conditions Long-pec posterior lateral line ganglion glial cell ISH
Long-pec trigeminal ganglion glial cell ISH
sox10m241/+ + MO3-disc1 standard conditions Long-pec posterior lateral line ganglion glial cell ISH
Long-pec trigeminal ganglion glial cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-disc1 standard conditions Long-pec posterior lateral line ganglion glial cell increased amount, abnormal
Long-pec trigeminal ganglion glial cell increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development