ZFIN ID: ZDB-FIG-090804-44
Drerup et al., 2009 - Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation. Development (Cambridge, England)   136(15):2623-2632 Full text @ Development
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 2 Two splice-site targeted Disc1 morpholinos altered disc1 transcript size and craniofacial development. (A) RT-PCR analysis of MOE3-injected embryos demonstrated alternative splicing of disc1 pre-mRNA resulting in a smaller transcript. At 6 hpf, maternally expressed disc1 could not be modified by the splice site-targeted morpholino, which resulted in the wild-type transcript present. (B) MOE8 injection also effectively altered transcript size. (C-H) Morpholino-mediated Disc1 knockdown resulted in three classes of craniofacial cartilage defects. (C,F) Class 1 (normal); (D,G) class 2; (E,H) class 3. (C-E) Lateral views; (F-H) ventral views with anterior to the left. (I) The ratio of MOE3 and MOE8 morphant embryos in each class was dose dependent. (I, right) Although half-effective doses of MOE3 or MOE8 did not affect craniofacial development, they acted synergistically to produce a high ratio of larvae with abnormal craniofacial cartilage.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-disc1 standard conditions Day 5 cranial cartilage malformed, abnormal
WT + MO4-disc1 standard conditions Day 5 cranial cartilage malformed, abnormal
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development