ZFIN ID: ZDB-FIG-090804-44
Drerup et al., 2009 - Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation. Development (Cambridge, England)   136(15):2623-2632 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 2 Two splice-site targeted Disc1 morpholinos altered disc1 transcript size and craniofacial development. (A) RT-PCR analysis of MOE3-injected embryos demonstrated alternative splicing of disc1 pre-mRNA resulting in a smaller transcript. At 6 hpf, maternally expressed disc1 could not be modified by the splice site-targeted morpholino, which resulted in the wild-type transcript present. (B) MOE8 injection also effectively altered transcript size. (C-H) Morpholino-mediated Disc1 knockdown resulted in three classes of craniofacial cartilage defects. (C,F) Class 1 (normal); (D,G) class 2; (E,H) class 3. (C-E) Lateral views; (F-H) ventral views with anterior to the left. (I) The ratio of MOE3 and MOE8 morphant embryos in each class was dose dependent. (I, right) Although half-effective doses of MOE3 or MOE8 did not affect craniofacial development, they acted synergistically to produce a high ratio of larvae with abnormal craniofacial cartilage.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO3-disc1 standard conditions Day 5 cranial cartilage malformed, abnormal
WT + MO4-disc1 standard conditions Day 5 cranial cartilage malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development