ZFIN ID: ZDB-FIG-090804-47
Drerup et al., 2009 - Disc1 regulates foxd3 and sox10 expression, affecting neural crest migration and differentiation. Development (Cambridge, England)   136(15):2623-2632 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: 10-13 somites to 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 10-13 somites to 20-25 somites

Fig. 5 CNC migration from the neural rod was hindered in Disc1 morphants. (A) DIC overlay showing a flat-mounted Tg(sox10:egfp) zebrafish (dorsal view, anterior to the left), demonstrating the four streams analyzed. (A-L) Flat-mounted control and Disc1 morphant Tg(sox10:egfp) zebrafish (dorsal view, anterior to the left) were fixed at evenly spaced intervals from 12s to 22s. CNC migratory distance was measured in the anterior (A), arch 1 (1), arch 2 (2) and arch 3 (3) streams of the neural crest (as indicated by the boxed regions in A). Vertical line marks the posterior eye; dotted lines outline the neural rod; curved lines outline the developing ear. (M-P) Graphical representation of the maximum migratory distance achieved by neural crest cells in each stream. Disc1 morphant CNC cells are hindered in their migration away from the dorsolateral midbrain and hindbrain. Height of the box is the mean±s.e.m. Error bars represent s.d.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP ba2Tg/+ control 10-13 somites to 20-25 somites cranial neural crest IHC
ba2Tg/+ + MO3-disc1 standard conditions 10-13 somites to 20-25 somites cranial neural crest IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ba2Tg/+ + MO3-disc1 standard conditions 10-13 somites - 20-25 somites neural crest cell migration disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development