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ZFIN ID: ZDB-FIG-080829-35
Hsu et al., 2006 - Mosaic Eyes is a novel component of the Crumbs complex and negatively regulates photoreceptor apical size. Development (Cambridge, England)   133(24):4849-4859 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth

Fig. 2 Localization of Moe and Crumbs proteins in embryonic brain and eye. (A) Western blot of wild type, moeb476, moeb781, moeb882 3d larvae probed with rabbit anti-Moe. Anti-Moe recognizes a protein of approximately 110 kDa in wild-type extracts that is absent in moe mutants. The blot was reprobed with anti-α-Tubulin as a loading control. (B) Anti-CRB3 recognizes the intracellular domains of all zebrafish Crumbs proteins. Western blot of GST, GST-Crb1intra, GST-Crb2aintra, GST-Crb2bintra, GST-Crb3aintra, GST-Crb3bintra probed with anti-CRB3 antibodies. The blot was reprobed with anti-GST as a loading control. (C-N) Moe (green) and panCrb (red) labeling in 30 hpf brain and eye in wild-type (C,D), moeb781 (E,F), crb2a morphant (G,H), nok (I,J), has (K,L) and ome (M,N) embryos. Arrows indicate brain ventricles and eyes are outlined. High magnification of the apical ventricular surface of the brain (BVS) and eye/retinal surface (ES) are shown to the right. Scale bars: 10 μm. (O) Western blot of 30 hpf wild-type, moeb781, crb2a morphant, nok and ome embryos probed with anti-panCrb antibodies. One-fifth of the material loaded for the panCrb Western was probed with anti-α-Tubulin to show relative loading amounts.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
crb1 Ab1-crb AB standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb AB + MO3-crb2a + MO4-crb2a standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb crb2am98/m98 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb epb41l5b781/b781 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb mpp5am520/m520 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb prkcim567/m567 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
crb2a Ab1-crb AB standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb Prim-15 whole organism WB
Ab1-crb AB + MO3-crb2a + MO4-crb2a standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb Prim-15 whole organism WB
Ab1-crb crb2am98/m98 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb Prim-15 Not Detected whole organism WB
Ab1-crb epb41l5b781/b781 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb Prim-15 whole organism WB
Ab1-crb mpp5am520/m520 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb Prim-15 whole organism WB
Ab1-crb prkcim567/m567 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
crb2b Ab1-crb AB standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb AB + MO3-crb2a + MO4-crb2a standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb crb2am98/m98 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb epb41l5b781/b781 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb mpp5am520/m520 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb prkcim567/m567 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
crb3a Ab1-crb AB standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb AB + MO3-crb2a + MO4-crb2a standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb crb2am98/m98 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb epb41l5b781/b781 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb mpp5am520/m520 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb prkcim567/m567 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
crb3b Ab1-crb AB standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb AB + MO3-crb2a + MO4-crb2a standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb crb2am98/m98 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb epb41l5b781/b781 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb mpp5am520/m520 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
Ab1-crb prkcim567/m567 standard conditions Prim-15 brain IHC
Ab1-crb Prim-15 eye IHC
epb41l5 Ab1-epb41l5 AB + MO3-crb2a + MO4-crb2a standard conditions Prim-15 brain IHC
Ab1-epb41l5 Prim-15 eye IHC
Ab1-epb41l5 WT standard conditions Prim-15 brain IHC
Ab1-epb41l5 Prim-15 eye IHC
Ab1-epb41l5 Protruding-mouth whole organism WB
Ab1-epb41l5 crb2am98/m98 standard conditions Prim-15 brain IHC
Ab1-epb41l5 Prim-15 eye IHC
Ab1-epb41l5 epb41l5b476/b476 standard conditions Protruding-mouth Not Detected whole organism WB
Ab1-epb41l5 epb41l5b781/b781 standard conditions Prim-15 Not Detected brain IHC
Ab1-epb41l5 Prim-15 Not Detected eye IHC
Ab1-epb41l5 Protruding-mouth Not Detected whole organism WB
Ab1-epb41l5 epb41l5b882/b882 standard conditions Protruding-mouth Not Detected whole organism WB
Ab1-epb41l5 mpp5am520/m520 standard conditions Prim-15 brain IHC
Ab1-epb41l5 Prim-15 eye IHC
Ab1-epb41l5 prkcim567/m567 standard conditions Prim-15 brain IHC
Ab1-epb41l5 Prim-15 eye IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development