ZFIN ID: ZDB-FIG-080402-19
Christensen et al., 2008 - Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development. BMC Developmental Biology   8:3 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-15

Fig. 3 Both the PDZ-binding domain and unique sequences in Epb4.1l5long required for rescue of moe- mutant defects. (A-C) At 30 hpf in wild-type embryos, Moe localizes cortically in brain and retinal neuroepithelial cells and is concentrated at the apical surface (A) and ZO-1 (green) and panCrb (red) localize to the apical surface of the retina (B) and brain (C). (D-F) At 30 hpf in moe- embryos, there is no Moe labeling (D) and ZO-1 (green) and panCrb (red) fail to localize to the apical surface of the retina (E) and brain (F). (G) At 30 hpf in moe- embryos injected with epb4.1l5long mRNA, Epb4.1l5long immunoreactivity is cortically localized in most retinal and brain neuroepithelial cells and ZO-1 (green) and panCrb (red) localize to the apical surface in the retina (H) and brain (I). Upper inset (G), magnified section of anti-Epb4.1l5long labeling and lower inset, uninjected moed ZO-1 (green) and panCrb (red) localize to the apical surface in the retina (H) and brain (I). Upper inset (G), magnified sect- embryos shows no labeling with anti-Epb4.1l5long. (J) At 30 hpf in moe- mutants injected with epb4.1l5short mRNA, anti-Epb4.1l5short is cytoplasmically localized and ZO-1 (green) and panCrb (red) do not localize to the apical surface in moe- retina (K) and brain (L). Upper inset (G), magnified section of anti-Epb4.1l5short labeling and lower inset, uninjected moe- embryos shows no labeling with anti-Epb4.1l5short. (M, N) At 30 hpf in moe- mutants injected with myc-epb4.1l5FERM mRNA, ZO-1 (green) and panCrb (red) do not localize to the apical surface in moe- retina (M) and brain (N). (O, P) At 30 hpf in moe- mutants injected with myc-epb4.1l5long_ΔPBD mRNA, ZO-1 (green) and panCrb (red) do not localize to the apical surface in moe- retina (O) and brain (P). (Q, R) At 30 hpf in moe- mutants injected with myc-epb4.1l5short+long_PBD mRNA, ZO-1 (green) and panCrb (red) localize to the apical surface in moe- retina (Q) and brain (R). (S, T) At 30 hpf in moe- mutants injected with myc-epb4.1l5long+short_PBD mRNA, ZO-1 (green) and panCrb (red) localize to the apical surface in moe- retina (S) and brain (T). (U) Western analysis of zebrafish embryos injected with epb4.1l5long and epb4.1l5short mRNA were tested for expression of protein product with isoform-specific antibodies at time points from 6 hpf to 96 hpf. (V) Western analysis of zebrafish embryos injected with epb4.1l5FERM (anti-Myc), epb4.1l5long_ΔPBD (anti-Epb4.1l5long), epb4.1l5short+long_PBD (anti-Myc), epb4.1l5long+short_PBD (anti-Epb4.1l5long). Blots stripped and reprobed with Anti-α-Tubulin as a loading control. Scale bars, 10 μm (A, D, G, J), 50 μm (lower insets in G, J), 10 μm (remaining panels). (A-T), single confocal z-sections.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
epb41l5 WT standard conditions Prim-15 brain IHC
Prim-15 retina IHC
epb41l5b781/b781 standard conditions Prim-15 Not Detected brain IHC
Prim-15 Not Detected retina IHC
tjp1a WT standard conditions Prim-15 brain IHC
Prim-15 retina IHC
epb41l5b781/b781 standard conditions Prim-15 brain IHC
Prim-15 retina IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
epb41l5b781/b781 standard conditions Prim-15 establishment or maintenance of epithelial cell apical/basal polarity process quality, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.