ZFIN ID: ZDB-FIG-080402-17
Christensen et al., 2008 - Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development. BMC Developmental Biology   8:3 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Pec-fin
PHENOTYPE:
Fish:
Observed In:
Stage: Pec-fin

Fig. 1 Injection of moe mRNA rescues defects in moe- embryos. (A-B) At 60 hpf, in wild-type embryos, the floor of the diencephalic ventricle is visible (A, white arrow head), the RPE is uniform, and the body axis is straight. (C, D) In moe- embryos, the ventricles are small or absent, the RPE is patchy, the tail curves and there is pericardial edema (D, arrow). (E, F) In moe- embryos injected with moe mRNA, the floor of the diencephalic ventricle is visible (E, white arrow head), the RPE is uniform, and the body axis is straight but mild pericardial edema persists (F, arrow). Anti-Moe labeling of 60 hpf wild-type embryos (G), moe- embryos (H), and moe- embryos injected with moe mRNA (I): the plexiform labeling in moe- embryos injected with moe mRNA (I, double arrowheads) is largely background. Adherens junctions (ZO-1, gnjected with moe mRNA (I): the plexiform labeling in moe- embryos injected with moe- embryos injected with moe mRNA (L), but are ectopically localized within the developing eye (arrow) and at the presumptive brain midline in moe- mutants with abnormal ventricle formation (asterix, K). High magnification confocal z-projections of TO-PRO-3 nuclear staining, and ZO-1 and panCrb labeling in the retina and brain in wild-type (M, N), moe- (O, P), and moe- embryos injected with moe mRNA (Q, R) at 60 hpf. (G, J, H, K, I and L are all single confocal z-sections). Scale bars, 50 μm (G-L), 10 μm (M-R).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
epb41l5 WT standard conditions Pec-fin brain IHC
Pec-fin retina IHC
epb41l5b781/b781 standard conditions Pec-fin Not Detected brain IHC
Pec-fin Not Detected retina IHC
tjp1a WT standard conditions Pec-fin brain IHC
Pec-fin retina IHC
epb41l5b781/b781 standard conditions Pec-fin brain IHC
Pec-fin retina IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
epb41l5b781/b781 standard conditions Pec-fin embryonic retina morphogenesis in camera-type eye process quality, abnormal
Pec-fin establishment or maintenance of epithelial cell apical/basal polarity process quality, abnormal
Pec-fin heart edematous, abnormal
Pec-fin post-vent region curved dorsal, abnormal
Pec-fin retinal pigmented epithelium patchy, abnormal
Pec-fin third ventricle absent, abnormal
Pec-fin third ventricle decreased volume, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.