ZFIN ID: ZDB-FIG-080402-22
Christensen et al., 2008 - Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development. BMC Developmental Biology   8:3 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Day 6
PHENOTYPE:
Fish:
Observed In:
Stage: Day 6

Fig. 6 The outer limiting membrane (OLM) is not restored in moe- mutants by injection of epb4.1l5long mRNA. (A) In wild-type larvae at 6 dpf, Crumbs proteins localize just apical to the OLM, which is labeled by anti-ZO-1. (B) In moe- mutants, very little Crumbs protein is visible and ZO-1 labeling is disorganized. (C) In epb4.1l5long mRNA injected moe- mutants, streaks of panCrb labeling are visible, but an organized OLM is absent. Ultrastructural transmission electron microscopic analysis at 6 dpf in wild-type (D), moe- mutants (E) and epb4.1l5long mRNA injected moe- mutants (F) retinas. Electron dense outer segments are seen in all individuals. Insets, higher magnifications of rod outer segments showing regular disc stacking is present in all individuals (100,000X). RPE, retinal pigmented epithelium; OS, outer segments; IS, inner Segments; CB, cell body. (A-C) are confocal z-projections, scale bar 10 μm (D-F). Scale bars, 5 μm (D-F), 100 nm (insets).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP epb41l5b781/b781; fl1Tg standard conditions Day 6 retinal rod cell IFL
fl1Tg standard conditions Day 6 retinal rod cell IFL
tjp1a epb41l5b781/b781; fl1Tg standard conditions Day 6 retina IHC
fl1Tg standard conditions Day 6 outer limiting membrane IHC
Day 6 retina IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
epb41l5b781/b781; fl1Tg standard conditions Day 6 outer limiting membrane aplastic, abnormal
Acknowledgments:
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