ZFIN ID: ZDB-IMAGE-080402-18
Figures for Christensen et al., 2008

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Fig. 3 Both the PDZ-binding domain and unique sequences in Epb4.1l5long required for rescue of moe- mutant defects. (A-C) At 30 hpf in wild-type embryos, Moe localizes cortically in brain and retinal neuroepithelial cells and is concentrated at the apical surface (A) and ZO-1 (green) and panCrb (red) localize to the apical surface of the retina (B) and brain (C). (D-F) At 30 hpf in moe- embryos, there is no Moe labeling (D) and ZO-1 (green) and panCrb (red) fail to localize to the apical surface of the retina (E) and brain (F). (G) At 30 hpf in moe- embryos injected with epb4.1l5long mRNA, Epb4.1l5long immunoreactivity is cortically localized in most retinal and brain neuroepithelial cells and ZO-1 (green) and panCrb (red) localize to the apical surface in the retina (H) and brain (I). Upper inset (G), magnified section of anti-Epb4.1l5long labeling and lower inset, uninjected moed ZO-1 (green) and panCrb (red) localize to the apical surface in the retina (H) and brain (I). Upper inset (G), magnified sect- embryos shows no labeling with anti-Epb4.1l5long. (J) At 30 hpf in moe- mutants injected with epb4.1l5short mRNA, anti-Epb4.1l5short is cytoplasmically localized and ZO-1 (green) and panCrb (red) do not localize to the apical surface in moe- retina (K) and brain (L). Upper inset (G), magnified section of anti-Epb4.1l5short labeling and lower inset, uninjected moe- embryos shows no labeling with anti-Epb4.1l5short. (M, N) At 30 hpf in moe- mutants injected with myc-epb4.1l5FERM mRNA, ZO-1 (green) and panCrb (red) do not localize to the apical surface in moe- retina (M) and brain (N). (O, P) At 30 hpf in moe- mutants injected with myc-epb4.1l5long_ΔPBD mRNA, ZO-1 (green) and panCrb (red) do not localize to the apical surface in moe- retina (O) and brain (P). (Q, R) At 30 hpf in moe- mutants injected with myc-epb4.1l5short+long_PBD mRNA, ZO-1 (green) and panCrb (red) localize to the apical surface in moe- retina (Q) and brain (R). (S, T) At 30 hpf in moe- mutants injected with myc-epb4.1l5long+short_PBD mRNA, ZO-1 (green) and panCrb (red) localize to the apical surface in moe- retina (S) and brain (T). (U) Western analysis of zebrafish embryos injected with epb4.1l5long and epb4.1l5short mRNA were tested for expression of protein product with isoform-specific antibodies at time points from 6 hpf to 96 hpf. (V) Western analysis of zebrafish embryos injected with epb4.1l5FERM (anti-Myc), epb4.1l5long_ΔPBD (anti-Epb4.1l5long), epb4.1l5short+long_PBD (anti-Myc), epb4.1l5long+short_PBD (anti-Epb4.1l5long). Blots stripped and reprobed with Anti-α-Tubulin as a loading control. Scale bars, 10 μm (A, D, G, J), 50 μm (lower insets in G, J), 10 μm (remaining panels). (A-T), single confocal z-sections.

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