ZFIN ID: ZDB-FIG-080402-20
Christensen et al., 2008 - Tissue-specific requirements for specific domains in the FERM protein Moe/Epb4.1l5 during early zebrafish development. BMC Developmental Biology   8:3 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Pec-fin to Day 4

Fig. 4 Morphological rescue of some moe- embryonic defects by injection of the epb4.1l5 constructs. (A) In wild-type embryos at 60 hpf, brain ventricles are visible, and the RPE is uniform (inset). (B) In moe- embryos, brain ventricles are reduced in size or absent, pericaridal edema is pronounced, and the RPE is patchy (inset). (C) In moe- embryos injected with epb4.1l5short+long_PBD mRNA, brain ventricles are reduced in size or absent, pericaridal edema is pronounced, but the RPE is normal (inset). (D) moe- embryos injected with epb4.1l5long+short_PBD mRNA, brain ventricles are absent or absent and pericardial edema is pronounced, and RPE defects are milder than those in uninjected moe- embryos. (E) A magnified view of the RPE of a 60 hpf wild-type embryo shows that it is uniform and the cells are confluent. (F) In a wild-typounced, and RPE defects are milder than those in uninjected moe- embryos. (E) A magnified view of the Re retina at 4 dpf, GFP+ rods localize next to the RPE and lamination is apparent. In 60 hpf moe- mutants, the integrity of the RPE varies from mild (G), to moderate (I), to severe (H). However, GFP+ rods are mislocalized in all moe- mutants 4 dpf (H, J, L). The integrity of the RPE is improved and nearly normal in a 60 hpf moe- mutant injected with epb4.1l5long+short_PBD mRNA (M) and most GFP+ rods are adjacent to the RPE (N). (O) A wild-type embryo injected with epb4.1l5long+short_PBD showing brain ventricles that are reduced or absent. (P) At 30 hpf Epb4.1l5long+short_PBD is cortically localized, upper inset is a 2x magnification of Epb4.1l5long+short_PBD localization. Scale bars, 10 μm (F), 50 μm (lower insets in F).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP epb41l5b781/b781; fl1Tg standard conditions Day 4 retinal rod cell IFL
fl1Tg standard conditions Day 4 retinal rod cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
epb41l5b781/b781 standard conditions Pec-fin retinal pigmented epithelium patchy, abnormal
epb41l5b781/b781; fl1Tg standard conditions Day 4 retinal rod cell mislocalised, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.