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ZFIN ID: ZDB-FIG-080401-15
Ignatius et al., 2008 - colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis. Developmental Biology   313(2):568-583 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage: Long-pec

Fig. 3 Melanophore development does not recover in hdac1col mutants. All panels are lateral views of 48 hpf embryos that are stained by in situ hybridization to reveal expression of mifta (A, B), c-kit (C, D), dct (E, F), fms (G, H) and xdh (I, J). (A, B) By 48 hpf mitfa expression is switched off in differentiating melanoblasts in wild-type; however in hdac1col mutants, mitfa continues to be expressed robustly in melanoblasts. (A, B, E, and F) There are still reduced numbers of mitfa and dct expressing melanoblasts in hdac1col mutants as compared to wild-type. Additionally, there is still a migration defect as most of the mitfa and dct expressing melanoblasts are located at their site of origin in the post otic region (arrowhead) and in the dorsal stripe (arrow). (C, D) In contrast to levels of mitfa and dct expression in melanoblasts in hdac1col mutants, c-kit expression in melanoblasts does not recover to wild-type levels (arrowheads). (G–J) Unlike melanophore development, xanthophore number and migration recover by 48 hpf, where there are numerous migrating fms-positive and xdh-positive xanthoblasts in wild-type and hdac1col mutants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aox5 WT standard conditions Long-pec xanthophore ISH
hdac1b382/b382 standard conditions Long-pec xanthophore ISH
csf1ra WT standard conditions Long-pec xanthophore ISH
hdac1b382/b382 standard conditions Long-pec xanthophore ISH
dct WT standard conditions Long-pec melanoblast ISH
hdac1b382/b382 standard conditions Long-pec melanoblast ISH
kita WT standard conditions Long-pec cloaca ISH
Long-pec melanoblast ISH
hdac1b382/b382 standard conditions Long-pec cloaca ISH
Long-pec melanoblast ISH
mitfa WT standard conditions Long-pec melanoblast ISH
hdac1b382/b382 standard conditions Long-pec melanoblast ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hdac1b382/b382 standard conditions Long-pec xanthophore amount, normal
Long-pec xanthophore cellular motility, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 313(2), Ignatius, M.S., Moose, H.E., El-Hodiri, H.M., and Henion, P.D., colgate/hdac1 repression of foxd3 expression is required to permit mitfa-dependent melanogenesis, 568-583, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.