Fig. 5

foxd3 expression is prolonged in the premigratory neural crest and increased numbers of cranial satellite glia are present in hdac1^col mutants. In situ hybridizations of sox10 (A, B) and foxd3 (C–J) between 24 hpf and 52 hpf. (A, B) Lateral views of 52 hpf embryos. At 52 hpf sox10 continues to be expressed robustly in the dorsal stripe in hdac1^col mutants, while expression in wild-type is absent to faint (arrowheads). (C, D) Lateral views of 24 hpf embryos. There is a prolonged expression of foxd3 in the premigratory neural crest cells at 24 hpf in hdac1^col mutants compared to wild-type (D, arrowheads). In wild-type, foxd3-positive neural crest cells are mostly present at the tip of the tail (C, arrowhead). In addition to increased numbers of neural crest cells expressing foxd3, foxd3 expression is also increased in the somites in hdac1eural crest cells are mostly present at the tip of the tail (C, arrowhead). In addition to increased numbers of neural crest cells expressing %^col mutants when compared to wild-type. (E, F) Flat mounts of the cranial region of 24 hpf embryos indicate that there is an increase in the number of cranial satellite glia in the pre otic and post otic placodes (arrowheads) in hdac1^col mutants as compared to wild-type. In contrast, pineal gland foxd3 expression (arrows) in hdac1^col mutants is slightly reduced compared to wild-type. (G, H) Lateral views of the tail region at 36 hpf. While foxd3 is not expressed in the premigratory neural crest cells in wild-type, it is still expressed in this population in hdac1^col mutants (arrowhead). (I, J) Lateral views of the head at 48 hpf. The number of foxd3-positive cranial satellite glia associated with the cranial ganglia is much larger than those present in wild-type embryos (black arrow and white arrowheads).