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ZFIN ID: ZDB-FIG-071019-38
Pei et al., 2007 - An early requirement for maternal FoxH1 during zebrafish gastrulation. Developmental Biology   310(1):10-22 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 75%-epiboly
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Shield to 14-19 somites

Fig. 6 Co-depletion of keratins phenocopies the FoxH1 morphant phenotype. (A–D) Morphological similarity of FoxH1 morphants and keratin morphants. Wild type embryos were injected with 8 ng of FoxH1 MO1, 16 ng of control MO, or 16 ng of a mixture containing 8 ng, 2 ng, 2 ng, 2 ng and 2 ng of MOs respectively targeting the translation of Cyt1, Cyt2, Keratin 4, Keratin 8 and Keratin 18. Un-injected (Uninj.) embryos are also shown to illustrate the moderate delays associated with a high dose (16 ng) of control MO. Injected embryos were kept in their chorions and placed into conical wells formed in 2% agarose, then simultaneously time lapse documented. Primes (′) indicate different time points for the same embryos. Most views are lateral, however embryos were free to rotate during the time lapse and panel A′ is closer to a frontal or ventral view. (E–P) Co-depletion of keratins phenocopies marker expression in FoxH1 morphants. Wild type embryos were injected with 8 ng of FoxH1 MO1, 16 ng of control MO, or 16 ng of the keratin MO mixture mentioned above and fixed at 8 hpf, when control embryos reached 70% epiboly, for in situ analysis. Panels E–H are dorsal views, and panels I–P, for which the dorsal side could not be determined, are randomly oriented side views. Abbreviations: FH1, FoxH1; ctrl, control.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyt1 WT control 75%-epiboly EVL ISH
WT + MO1-cyt1 + MO1-krt18 + MO1-krt4 + MO1-krt5 + MO1-krt8 standard conditions 75%-epiboly EVL ISH
WT + MO1-foxh1 standard conditions 75%-epiboly EVL ISH
pax2a WT control 75%-epiboly presumptive midbrain hindbrain boundary ISH
WT + MO1-cyt1 + MO1-krt18 + MO1-krt4 + MO1-krt5 + MO1-krt8 standard conditions 75%-epiboly presumptive midbrain hindbrain boundary ISH
WT + MO1-foxh1 standard conditions 75%-epiboly presumptive midbrain hindbrain boundary ISH
snai1a WT control 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-cyt1 + MO1-krt18 + MO1-krt4 + MO1-krt5 + MO1-krt8 standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
WT + MO1-foxh1 standard conditions 75%-epiboly presumptive paraxial mesoderm ISH
sox17 WT control 75%-epiboly endoderm ISH
WT + MO1-cyt1 + MO1-krt18 + MO1-krt4 + MO1-krt5 + MO1-krt8 standard conditions 75%-epiboly endoderm ISH
WT + MO1-foxh1 standard conditions 75%-epiboly endoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cyt1 + MO1-krt18 + MO1-krt4 + MO1-krt5 + MO1-krt8 standard conditions Shield cell migration involved in gastrulation disrupted, abnormal
14-19 somites whole organism dead, abnormal
WT + MO1-foxh1 standard conditions Shield cell migration involved in gastrulation disrupted, abnormal
14-19 somites whole organism dead, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 310(1), Pei, W., Noushmehr, H., Costa, J., Ouspenskaia, M.V., Elkahloun, A.G., and Feldman, B., An early requirement for maternal FoxH1 during zebrafish gastrulation, 10-22, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.