ZFIN ID: ZDB-FIG-070307-34
Nechiporuk et al., 2007 - Specification of epibranchial placodes in zebrafish. Development (Cambridge, England)   134(3):611-623 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Bud to Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage Range: 14-19 somites to Prim-5

Fig. 5 Fgf3 and Fgf8 are necessary for EB placode induction. (A) fgf3+/-;fgf8+/- embryos were crossed to generate various genotypic combinations, including fgf3;fgf8 mutants. Resulting embryos were processed for in-situ hybridization with eya1, foxi1, pax2a, ngn1 and phox2b riboprobes, photographed and genotyped (genotypes are shown in the bottom left of each panels). All panels show lateral views, except eya1-expression panels, which show dorsal views. PPE is not affected in fgf3 or fgf8 mutants or fgf3;fgf8 double mutant. Consistent with our previous observations (Nechiporuk et al., 2005), fgf3 mutants lacked ngn1 and phox2b expression in glossopharyngeal and small vagal ganglia, whereas foxi1 and pax2a expression was normal. All markers were expressed normally in fgf8-/- embryos. However, foxi1 expression (brackets) was strongly reduced and pax2a, ngn1, and phox2b expression was either absent or strongly reduced in fgf3;fgf8 double mutants. Vagal neural crest is not affected in fgf3;fgf8 double mutants (arrowheads). fgf3+/-;fgf8-/-embryos displayed intermediate phenotypes, where expression of all markers were reduced but not absent. (B,C) Transverse sections of fgf3/8 double mutants and wild-type siblings were obtained from the whole-mounts processed for foxi1 (B) and pax2a (C) in situ (dashed lines in A indicate level of cross section). In contrast to wild type, the epithelium appears disorganized and foxi1 expression is not restricted to a single cell layer in fgf3;fgf8 mutants. pax2a expression is absent from the ectoderm in fgf3;fgf8 mutants. Scale bars: 50 μm. Abbreviations are as in Fig. 1; a, acoustic ganglion; al, anterior lateral line ganglion; op, otic placode.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
eya1 WT control Bud unspecified ISH
fgf3t24149/+; fgf8ati282a/ti282a standard conditions Bud unspecified ISH
fgf3t24149/t24149; fgf8ati282a/+ standard conditions Bud unspecified ISH
fgf3t24149/t24149; fgf8ati282a/ti282a standard conditions Bud unspecified ISH
fgf8ati282a/ti282a standard conditions Bud unspecified ISH
foxi1 fgf3t24149/+; fgf8ati282a/+ standard conditions 20-25 somites unspecified ISH
fgf3t24149/+; fgf8ati282a/ti282a standard conditions 14-19 somites neurogenic placode ISH
fgf3t24149/t24149; fgf8ati282a/+ standard conditions 14-19 somites neurogenic placode ISH
fgf3t24149/t24149; fgf8ati282a/ti282a standard conditions 14-19 somites neurogenic placode ISH
20-25 somites unspecified ISH
fgf8ati282a/+ standard conditions 14-19 somites neurogenic placode ISH
fgf8ati282a/ti282a standard conditions 14-19 somites neurogenic placode ISH
neurog1 fgf3t24149/+; fgf8ati282a/+ standard conditions Prim-25 anterior lateral line ganglion ISH
Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 statoacoustic (VIII) ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 4 ISH
fgf3t24149/+; fgf8ati282a/ti282a standard conditions Prim-25 anterior lateral line ganglion ISH
Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 statoacoustic (VIII) ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 4 ISH
fgf3t24149/t24149; fgf8ati282a/+ standard conditions Prim-25 anterior lateral line ganglion ISH
Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 statoacoustic (VIII) ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 4 ISH
fgf3t24149/t24149; fgf8ati282a/ti282a standard conditions Prim-25 anterior lateral line ganglion ISH
Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 statoacoustic (VIII) ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 3 ISH
Prim-25 vagal ganglion 4 ISH
fgf8ati282a/ti282a standard conditions Prim-25 anterior lateral line ganglion ISH
Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 statoacoustic (VIII) ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 4 ISH
pax2a fgf3t24149/+; fgf8ati282a/+ standard conditions Prim-5 unspecified ISH
fgf3t24149/+; fgf8ati282a/ti282a standard conditions 14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
Prim-5 glossopharyngeal placode ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
Prim-5 vagal ganglion 1 ISH
Prim-5 vagal ganglion 2 ISH
Prim-5 vagal ganglion 3 ISH
Prim-5 vagal ganglion 4 ISH
fgf3t24149/t24149 standard conditions 14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
fgf3t24149/t24149; fgf8ati282a/+ standard conditions Prim-5 glossopharyngeal placode ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
Prim-5 vagal ganglion 1 ISH
Prim-5 vagal ganglion 2 ISH
Prim-5 vagal ganglion 3 ISH
Prim-5 vagal ganglion 4 ISH
fgf3t24149/t24149; fgf8ati282a/ti282a standard conditions 14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
Prim-5 glossopharyngeal placode ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
Prim-5 vagal ganglion 1 ISH
Prim-5 vagal ganglion 2 ISH
Prim-5 vagal ganglion 3 ISH
Prim-5 vagal ganglion 4 ISH
fgf8ati282a/+ standard conditions 14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
Prim-5 glossopharyngeal placode ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
Prim-5 vagal ganglion 1 ISH
Prim-5 vagal ganglion 2 ISH
Prim-5 vagal ganglion 3 ISH
Prim-5 vagal ganglion 4 ISH
fgf8ati282a/ti282a standard conditions 14-19 somites midbrain hindbrain boundary ISH
14-19 somites otic placode ISH
Prim-5 glossopharyngeal placode ISH
Prim-5 midbrain hindbrain boundary ISH
Prim-5 otic vesicle ISH
Prim-5 vagal ganglion 1 ISH
Prim-5 vagal ganglion 2 ISH
Prim-5 vagal ganglion 3 ISH
Prim-5 vagal ganglion 4 ISH
phox2bb fgf3t24149/+ standard conditions Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 3 ISH
Prim-25 vagal ganglion 4 ISH
fgf3t24149/+; fgf8ati282a/ti282a standard conditions Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 3 ISH
Prim-25 vagal ganglion 4 ISH
fgf3t24149/t24149; fgf8ati282a/+ standard conditions Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 3 ISH
Prim-25 vagal ganglion 4 ISH
fgf3t24149/t24149; fgf8ati282a/ti282a standard conditions Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 3 ISH
Prim-25 vagal ganglion 4 ISH
fgf8ati282a/ti282a standard conditions Prim-25 facial ganglion ISH
Prim-25 glossopharyngeal ganglion ISH
Prim-25 vagal ganglion 1 ISH
Prim-25 vagal ganglion 2 ISH
Prim-25 vagal ganglion 3 ISH
Prim-25 vagal ganglion 4 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
fgf3t24149/+; fgf8ati282a/ti282a standard conditions 14-19 somites midbrain hindbrain boundary morphology, abnormal
14-19 somites midbrain-hindbrain boundary development disrupted, abnormal
fgf3t24149/t24149; fgf8ati282a/+ standard conditions Prim-5 epibranchial placode morphology, abnormal
Prim-5 peripheral nervous system development disrupted, abnormal
fgf3t24149/t24149; fgf8ati282a/ti282a standard conditions 14-19 somites ectoderm morphology, abnormal
14-19 somites ectoderm development disrupted, abnormal
14-19 somites epithelium disorganized, abnormal
14-19 somites midbrain hindbrain boundary morphology, abnormal
14-19 somites midbrain-hindbrain boundary development disrupted, abnormal
14-19 somites morphogenesis of embryonic epithelium disrupted, abnormal
14-19 somites otic vesicle aplastic, abnormal
14-19 somites otic vesicle formation disrupted, abnormal
Prim-5 epibranchial placode morphology, abnormal
Prim-5 peripheral nervous system development disrupted, abnormal
fgf8ati282a/ti282a standard conditions 14-19 somites midbrain hindbrain boundary morphology, abnormal
14-19 somites midbrain-hindbrain boundary development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development