PUBLICATION

Specification of epibranchial placodes in zebrafish

Authors
Nechiporuk, A., Linbo, T., Poss, K.D., and Raible, D.W.
ID
ZDB-PUB-070122-31
Date
2007
Source
Development (Cambridge, England)   134(3): 611-623 (Journal)
Registered Authors
Linbo, Tor, Nechiporuk, Alex, Poss, Kenneth D., Raible, David
Keywords
Fgf3, Fgf8, Foxi1, Pax2a, Epibranchial placodes, Cranial ganglia, Cephalic mesoderm, Zebrafish
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Branchial Region/embryology*
  • Branchial Region/metabolism
  • Fibroblast Growth Factor 3/genetics
  • Fibroblast Growth Factor 3/metabolism
  • Fibroblast Growth Factors/genetics
  • Fibroblast Growth Factors/metabolism
  • Forkhead Transcription Factors/genetics
  • Forkhead Transcription Factors/metabolism
  • Gene Expression Regulation, Developmental
  • Models, Neurological
  • Mutation
  • Oligodeoxyribonucleotides, Antisense/genetics
  • PAX2 Transcription Factor/genetics
  • PAX2 Transcription Factor/metabolism
  • Signal Transduction
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
PubMed
17215310 Full text @ Development
Abstract
In all vertebrates, the neurogenic placodes are transient ectodermal thickenings that give rise to sensory neurons of the cranial ganglia. Epibranchial (EB) placodes generate neurons of the distal facial, glossopharyngeal and vagal ganglia, which convey sensation from the viscera, including pharyngeal endoderm structures, to the CNS. Recent studies have implicated signals from pharyngeal endoderm in the initiation of neurogenesis from EB placodes; however, the signals underlying the formation of placodes are unknown. Here, we show that zebrafish embryos mutant for fgf3 and fgf8 do not express early EB placode markers, including foxi1 and pax2a. Mosaic analysis demonstrates that placodal cells must directly receive Fgf signals during a specific crucial period of development. Transplantation experiments and mutant analysis reveal that cephalic mesoderm is the source of Fgf signals. Finally, both Fgf3 and Fgf8 are sufficient to induce foxi1-positive placodal precursors in wild-type as well as Fgf3-plus Fgf8-depleted embryos. We propose a model in which mesoderm-derived Fgf3 and Fgf8 signals establish both the EB placodes and the development of the pharyngeal endoderm, the subsequent interaction of which promotes neurogenesis. The coordinated interplay between craniofacial tissues would thus assure proper spatial and temporal interactions in the shaping of the vertebrate head.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes