ZFIN ID: ZDB-FIG-060322-5
Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation. Developmental Biology   290(1):152-163 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 Inhibition of gsc and foxA3 results in an upregulation of Wnt signaling at the dorsal margin. Compared to wild-type (A), embryos injected with wnt8a DNA (B) or inactivation of dkk1 by morpholino knock-down (C) results in deletion of anterior head. (D–F) Expression of wnt8a at shield stage in WT (D), gsc (E) and gsc/foxa3 (F) knock-down embryos. Arrowheads indicate the dorsal borders of wnt8a expression territory. (G–I) Expression of frzb at shield stage in WT (G), gsc (H) and gsc/foxa3 (I) knock-down embryos. (J, K) Expression of dkk1 at shield stage in WT (J) and gsc/foxa3 knock-down (K) embryos. Arrows indicate the dorsal most extent domain of dkk1 expression at the margin. (L) Expression of wnt8a at shield stage in an embryo injected with gsc RNA at the 32 cell stage in one ventro-lateral blastomere. Arrowheads indicate the limit of wnt8a expression at the gastrula margin. (A, B) 96 hpf, (C) 48 hpf, (D–L) shield stage. (A–C, G–I) Lateral view, (D–F) dorsal view, (J–L) animal pole view dorsal to the right.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dkk1b WT standard conditions Shield margin ISH
WT + MO1-foxa3 + MO1-gsc standard conditions Shield margin ISH
frzb WT standard conditions Shield margin ISH
WT + MO1-foxa3 + MO1-gsc standard conditions Shield Not Detected margin ISH
WT + MO1-gsc standard conditions Shield margin ISH
wnt8a WT standard conditions Shield margin ISH
WT + MO1-foxa3 + MO1-gsc standard conditions Shield margin ISH
WT + MO1-gsc standard conditions Shield margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dkk1b standard conditions Long-pec head morphogenesis disrupted, abnormal
Long-pec head truncated, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.