ZFIN ID: ZDB-FIG-060322-6
Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation. Developmental Biology   290(1):152-163 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 6 Inhibition of Wnt8a signaling rescues the headless phenotype of gsc/foxA3 knock-down embryos. Expression of the telencephalic marker emx1 in wild-type embryo (A), in double gsc/foxa3 knock-down embryo (B), in gsc/foxa3 knock-down embryo coinjected with either frzb RNA (C), dkk1 RNA (D) or wnt8a Mo (E). (F–J) Morphological analysis of embryos treated with the same conditions of injections than in panels A–E showing rescue of the head at 32 hpf when the Wnt signaling pathway is inhibited. (K) Frequency of the phenotypic classes observed for the abovementioned injections. (A–E) 2–3 somite stage, dorsal views; (F–J) lateral views at 32 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
emx1 WT standard conditions 1-4 somites presumptive telencephalon ISH
WT + MO1-foxa3 + MO1-gsc standard conditions 1-4 somites Not Detected presumptive telencephalon ISH
WT + MO1-foxa3 + MO1-gsc + MO1-wnt8a standard conditions 1-4 somites presumptive telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxa3 + MO1-gsc standard conditions Prim-5 eye fused with eye, abnormal
Prim-5 head truncated, abnormal
Prim-15 head morphogenesis disrupted, abnormal
Prim-15 head truncated, abnormal
WT + MO1-foxa3 + MO1-gsc + MO1-wnt8a standard conditions Prim-5 eye fused with eye, abnormal
Prim-5 head truncated, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.