ZFIN ID: ZDB-FIG-060322-3
Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation. Developmental Biology   290(1):152-163 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-25

Fig. 2 foxA3 inactivation enhances gsc loss-of-function phenotype. (A–F) Inactivation of gsc leads to head structure alterations ranging from cyclopia (class I, B and E) to a complete deletion of the anterior head (class II, C and F). (G–I) Morpholino knock-down of foxA3 results in an expansion of ventro-caudal mesenchyme (G and I) whereas the head is unaffected (H). (J–T) Double inactivation of gsc and foxA3 results in head truncation visible morphologically as a cyclopia (class I, K) or a complete deletion of anterior head (class II, L). Analysis after in situ hybridization with otx5 (epiphysis, blue, M–P) and eng2 (MHB, red, M–P) or (Q–T) with pax6 (eye, forebrain, blue) and eng2 (red) reveals much stronger phenotypes including complete loss of anterior structures from telencephalon up to MHB (class III). (U) The penetrance of class I and class II phenotypes analyzed at 24 hpf in Mo-gsc plus Mo-foxA3 injected embryos (20 pg each Mo) was also strongly enhanced compared to the Mo-foxA3 (20 pg) or Mo-gsc (20 pg) injected groups. (A–C; G–T) Lateral view, (D–F) front view. (A–F) Embryos at 36 hpf; (G–T) embryos at 24 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
en2a WT standard conditions Prim-5 midbrain hindbrain boundary ISH
WT + MO1-foxa3 + MO1-gsc standard conditions Prim-5 midbrain hindbrain boundary ISH
otx5 WT standard conditions Prim-5 epiphysis ISH
WT + MO1-foxa3 + MO1-gsc standard conditions Prim-5 epiphysis ISH
pax6a WT standard conditions Prim-5 forebrain ISH
Prim-5 immature eye ISH
WT + MO1-foxa3 + MO1-gsc standard conditions Prim-5 forebrain ISH
Prim-5 immature eye ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-foxa3 standard conditions Prim-5 head morphogenesis process quality, normal
Prim-5 tail bud morphology, abnormal
Prim-5 ventral mesenchyme distended, abnormal
WT + MO1-foxa3 + MO1-gsc standard conditions Prim-5 eye fused with eye, abnormal
Prim-5 forebrain aplastic, abnormal
Prim-5 head aplastic, abnormal
Prim-5 head morphogenesis disrupted, abnormal
Prim-5 midbrain aplastic, abnormal
Prim-5 midbrain hindbrain boundary aplastic, abnormal
WT + MO1-gsc standard conditions Prim-5 eye fused with eye, abnormal
Prim-5 head truncated, abnormal
Prim-25 eye fused with eye, abnormal
Prim-25 head hypoplastic, abnormal
Prim-25 head morphogenesis disrupted, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.