ZFIN ID: ZDB-FIG-060322-4
Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation. Developmental Biology   290(1):152-163 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 75%-epiboly

Fig. 4 Inhibition of gsc plus foxA3 posteriorizes the ectoderm. (A–F) Expression of the presumptive epidermal marker foxi1 and the presumptive MHB marker pax2.1 in WT (A, D), gsc (B, E) and gsc/foxA3 (C, F) knock-down embryos. The region between the animal pole (red star) and the presumptive MHB (arrowhead) corresponding to the presumptive forebrain and midbrain is reduced in gsc and almost absent in gsc/foxA3 knock-down embryos. (G, H) Expression of hoxa1 in WT (G) is expanded anteriorly (red arrow) in gsc/foxA3 knock-down embryos (H). The embryos are at 80% epiboly. (A–C, G and H left) Lateral views and (D–F) animal pole view, dorsal to the right. (G and H right) Dorsal views anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxi1 WT standard conditions 75%-epiboly ectoderm ISH
WT + MO1-foxa3 + MO1-gsc standard conditions 75%-epiboly ectoderm ISH
WT + MO1-gsc standard conditions 75%-epiboly ectoderm ISH
hoxb1b WT standard conditions 75%-epiboly presumptive neural plate ISH
WT + MO1-foxa3 + MO1-gsc standard conditions 75%-epiboly presumptive neural plate ISH
pax2a WT standard conditions 75%-epiboly presumptive midbrain hindbrain boundary ISH
WT + MO1-foxa3 + MO1-gsc standard conditions 75%-epiboly presumptive midbrain hindbrain boundary ISH
WT + MO1-gsc standard conditions 75%-epiboly presumptive midbrain hindbrain boundary ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.