ZFIN Figure: Seiliez et al., 2006, Fig. 4

ZFIN ID: ZDB-FIG-060322-4

ZFIN ID: ZDB-FIG-060322-4

Seiliez et al., 2006 - FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation. Developmental Biology 290(1):152-163 Full text @ Dev. Biol.

ADDITIONAL FIGURES

EXPRESSION / LABELING:

Genes:	foxi1 hoxb1b pax2a
Fish:	WT WT + MO1-gsc WT + MO1-foxa3 + MO1-gsc
Knockdown Reagents:	MO1-foxa3 MO1-gsc
Anatomical Terms:	ectoderm presumptive midbrain hindbrain boundary presumptive neural plate
Stage:	75%-epiboly

Fig. 4 Inhibition of gsc plus foxA3 posteriorizes the ectoderm. (A–F) Expression of the presumptive epidermal marker foxi1 and the presumptive MHB marker pax2.1 in WT (A, D), gsc (B, E) and gsc/foxA3 (C, F) knock-down embryos. The region between the animal pole (red star) and the presumptive MHB (arrowhead) corresponding to the presumptive forebrain and midbrain is reduced in gsc and almost absent in gsc/foxA3 knock-down embryos. (G, H) Expression of hoxa1 in WT (G) is expanded anteriorly (red arrow) in gsc/foxA3 knock-down embryos (H). The embryos are at 80% epiboly. (A–C, G and H left) Lateral views and (D–F) animal pole view, dorsal to the right. (G and H right) Dorsal views anterior to the top.

Gene Expression Details

Gene	Fish	Conditions	Stage	Anatomy	Assay
foxi1	WT	standard conditions	75%-epiboly	ectoderm	ISH
	WT + MO1-foxa3 + MO1-gsc	standard conditions	75%-epiboly	ectoderm	ISH
	WT + MO1-gsc	standard conditions	75%-epiboly	ectoderm	ISH
hoxb1b	WT	standard conditions	75%-epiboly	presumptive neural plate	ISH
	WT + MO1-foxa3 + MO1-gsc	standard conditions	75%-epiboly	presumptive neural plate	ISH
pax2a	WT	standard conditions	75%-epiboly	presumptive midbrain hindbrain boundary	ISH
	WT + MO1-foxa3 + MO1-gsc	standard conditions	75%-epiboly	presumptive midbrain hindbrain boundary	ISH
	WT + MO1-gsc	standard conditions	75%-epiboly	presumptive midbrain hindbrain boundary	ISH

Antibody Labeling Details No data available

Phenotype Details No data available

Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.

Acknowledgments:

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 290(1), Seiliez, I., Thisse, B., and Thisse, C., FoxA3 and goosecoid promote anterior neural fate through inhibition of Wnt8a activity before the onset of gastrulation, 152-163, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.