ZFIN ID: ZDB-FIG-060208-5
Seth et al., 2006 - belladonna/(lhx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain. Development (Cambridge, England)   133(4):725-735 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 6 The axon growth substrate is disrupted in bel mutants. (A, B) Confocal images of ventral views of 21 hpf embryos labeled with anti-GFAP antibody (red); white line marks the optic recess. (A) GFAP expressing glial cells span the midline in the position of the AC (arrow) and POC (arrowhead). (B) In bel mutants, these glial bridges are disorganized and glial cell bodies are present between the commissures (arrows). Insets show a similar disorganization of glial cells in the mutant at 28 hpf in embryos double labeled for axons (anti-AT, green) and glial cells (anti-GFAP, red). (C, D) sema3d is absent in the forebrain of the bel mutants (arrowhead). Upper insets show ventral views; lower insets show a similar loss of sema3d expression in the anterior diencephalon after injection of lhx2 antisense MO into a wild-type embryo. (E, F) netrin1a expression is expanded ventrally into the diencephalon in bel mutants (arrowhead). (G, H) Expression of slit2 in bel mutants is expanded across the chiasm region (arrows). Insets show ventral views, anterior up. (I, J) ephB2 expression is reduced in the diencephalon and hypothalamus (arrows), but appears normal in the chiasm region (arrowheads). (K-N) Schematic lateral (K,L) and ventral (M,N) views showing expression of a subset of the genes described in this and the previous figure. AC, anterior commissure; OR, optic recess; POC, post optic commissure; black dots mark the position of the optic recess.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
efnb2a WT control Prim-5 diencephalon ISH
Prim-5 hypothalamus ISH
lhx2bunspecified control Prim-5 diencephalon ISH
Prim-5 hypothalamus ISH
gfap WT standard conditions 20-25 somites brain IHC
Prim-5 brain IHC
lhx2bunspecified standard conditions 20-25 somites brain IHC
Prim-5 brain IHC
ntn1a WT control Prim-5 diencephalon ISH
lhx2bunspecified control Prim-5 diencephalon ISH
sema3d WT control Prim-5 diencephalon ISH
WT + MO2-lhx2b standard conditions Prim-5 Not Detected diencephalon ISH
lhx2bunspecified control Prim-5 Not Detected diencephalon ISH
slit2 WT control Prim-5 diencephalon ISH
lhx2bunspecified control Prim-5 diencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development