PUBLICATION

belladonna/(lhx2) is required for neural patterning and midline axon guidance in the zebrafish forebrain

Authors
Seth, A., Culverwell, J., Walkowicz, M., Toro, S., Rick, J.M., Neuhauss, S.C., Varga, Z.M., and Karlstrom, R.O.
ID
ZDB-PUB-060130-7
Date
2006
Source
Development (Cambridge, England)   133(4): 725-735 (Journal)
Registered Authors
Karlstrom, Rolf, Neuhauss, Stephan, Rick, Jens, Seth, Anandita, Toro, Sabrina, Varga, Zoltan M., Walkowicz, Mitch
Keywords
AC, Chiasm, Commissure, Ephrin, Gfap, Glial bridge, Morpholino, Netrin, POC, Semaphorin, Slit
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Axons/physiology*
  • Body Patterning*
  • Cell Proliferation
  • Diencephalon/embryology
  • Diencephalon/metabolism
  • Eye/cytology
  • Eye/embryology*
  • Fibroblast Growth Factors/metabolism
  • LIM-Homeodomain Proteins
  • Molecular Sequence Data
  • Morphogenesis
  • Mutation
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/physiology
  • Neuroglia/physiology
  • Prosencephalon/embryology*
  • Signal Transduction
  • Telencephalon/embryology
  • Telencephalon/metabolism
  • Transcription Factors
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
16436624 Full text @ Development
Abstract
Some of the earliest axon pathways to form in the vertebrate forebrain are established as commissural and retinal axons cross the midline of the diencephalon and telencephalon. To better understand axon guidance in the forebrain, we characterized the zebrafish belladonna (bel) mutation, which disrupts commissural and retinal axon guidance in the forebrain. Using a positional cloning strategy, we determined that the bel locus encodes zebrafish Lhx2, a lim-homeodomain transcription factor expressed in the brain, eye and fin buds. We show that bel(lhx2) function is required for patterning in the ventral forebrain and eye, and that loss of bel function leads to alterations in regulatory gene expression, perturbations in axon guidance factors, and the absence of an optic chiasm and forebrain commissures. Our analysis reveals new roles for lhx2 in midline axon guidance, forebrain patterning and eye morphogenesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes