ZFIN ID: ZDB-FIG-050509-3
Dutta et al., 2005 - pitx3 defines an equivalence domain for lens and anterior pituitary placode. Development (Cambridge, England)   132(7):1579-1590 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5

Fig. 5 pitx3 is required for pituitary placode formation, whereas dlx3b and dlx4b control pituitary placode size. Wild-type (A-C) and morpholino (MO)-injected (D-L) embryos labeled with probes for lim3 (A,D,G,J), nkx2.2 (B,E,H,K) and pomc (C,F,I,L). (D-F) Injection of pitx3 morpholinos (n=94) leads to loss of pituitary placode (20/94 embryos) or severe reduction in pituitary cell number (D, inset; n=69/94). (G-I) Injection of dlx3b and dlx4b morpholinos (n=103) leads to enlarged pituitary placodes and increased pituitary cell numbers (n=78/103), arrowheads in B,E,H. (J-L) Injection of pitx3, dlx3b and dlx4b morpholinos (n=83) leads to loss of pituitary placode (42/83 embryos) or severe reduction in pituitary cell number (n=25/83). (A,C,D,F,G,I,J,L) Frontal views, dorsal towards the top; (B,E,H,K) side views, anterior towards the left. Scale bars: 100 µm in A-L; 25 µm in D, inset.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lhx3 WT standard conditions Prim-5 hypophysis ISH
WT + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 hypophysis ISH
WT + MO1-dlx3b + MO1-dlx4b + MO3-pitx3 standard conditions Prim-5 Not Detected hypophysis ISH
WT + MO3-pitx3 standard conditions Prim-5 Not Detected hypophysis ISH
WT + MO3-pitx3 standard conditions Prim-5 hypophysis ISH
nkx2.2a WT standard conditions Prim-5 forebrain ISH
Prim-5 hypophysis ISH
WT + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 forebrain ISH
Prim-5 hypophysis ISH
WT + MO1-dlx3b + MO1-dlx4b + MO3-pitx3 standard conditions Prim-5 forebrain ISH
Prim-5 Not Detected hypophysis ISH
WT + MO3-pitx3 standard conditions Prim-5 forebrain ISH
Prim-5 Not Detected hypophysis ISH
pomca WT standard conditions Prim-5 hypophysis ISH
WT + MO1-dlx3b + MO1-dlx4b standard conditions Prim-5 hypophysis ISH
WT + MO1-dlx3b + MO1-dlx4b + MO3-pitx3 standard conditions Prim-5 Not Detected hypophysis ISH
WT + MO3-pitx3 standard conditions Prim-5 Not Detected hypophysis ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development