ZFIN ID: ZDB-FIG-050120-7
Little et al., 2004 - Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning. Development (Cambridge, England)   131(23):5825-5835 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: 5-9 somites

Fig. 3 Interaction between sub-dorsalizing tsg1 knockdown and swirl (bmp2b) heterozygotes. All embryos were injected with 16 ng tsg1 MO. At 24 hpf, an injected wild-type embryo was not dorsalized (A), whereas injected swirl (bmp2b) heterozygous siblings were dorsalized. The extent of dorsalization was categorized as follows: (B) weak, class 1 (arrowhead indicates partial loss of the ventral tail fin); (C) moderate, class 3; and (D) strong, class 4. (E-J) Whole-mount in situ hybridization of myod, pax2a and krox20 in 16 ng tsg1 MO1 injected embryos at the eight-somite stage. Injected wild-type embryos were not affected (E,F), whereas heterozygotes displayed a range of dorsalizations (G,H, moderate; I, strong; J, stronger). (F,H) More posterior views of the same embryos shown in E,G, respectively. (J) The somites extend around the circumference, which is typical of a strong, class 4 dorsalization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT + MO1-twsg1b standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
bmp2btc300a/tc300a + MO1-twsg1b (TU) standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
myod1 WT + MO1-twsg1b standard conditions 5-9 somites mesoderm ISH
bmp2btc300a/tc300a + MO1-twsg1b (TU) standard conditions 5-9 somites mesoderm ISH
pax2a WT + MO1-twsg1b standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
bmp2btc300a/tc300a + MO1-twsg1b (TU) standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development