ZFIN ID: ZDB-IMAGE-050120-7
Figures for Little et al., 2004

Genes:

Figure Caption/Comments:

OPTICS: MAGNIFICATION: DATE OF IMAGE: SUBMITTER COMMENTS:

Fig. 3 Interaction between sub-dorsalizing tsg1 knockdown and swirl (bmp2b) heterozygotes. All embryos were injected with 16 ng tsg1 MO. At 24 hpf, an injected wild-type embryo was not dorsalized (A), whereas injected swirl (bmp2b) heterozygous siblings were dorsalized. The extent of dorsalization was categorized as follows: (B) weak, class 1 (arrowhead indicates partial loss of the ventral tail fin); (C) moderate, class 3; and (D) strong, class 4. (E-J) Whole-mount in situ hybridization of myod, pax2a and krox20 in 16 ng tsg1 MO1 injected embryos at the eight-somite stage. Injected wild-type embryos were not affected (E,F), whereas heterozygotes displayed a range of dorsalizations (G,H, moderate; I, strong; J, stronger). (F,H) More posterior views of the same embryos shown in E,G, respectively. (J) The somites extend around the circumference, which is typical of a strong, class 4 dorsalization.

Figure Data:
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.