ZFIN ID: ZDB-FIG-050120-10
Little et al., 2004 - Twisted gastrulation promotes BMP signaling in zebrafish dorsal-ventral axial patterning. Development (Cambridge, England)   131(23):5825-5835 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 5-9 somites

Fig. 7 Overexpression of tsg1 RNA causes dorsalization. (A-E) Whole-mount in situ hybridization with pax2.1 (arrowhead), krox20 (brackets) and myod reveals expansion of all three markers in embryos injected with 200 pg tsg1 RNA (C-E) relative to wild type (A,B). (B,D) More posterior views of embryos shown in A and C, respectively. (C,D) Mildly dorsalized and (E) moderately dorsalized embryos. (F-I) At 1 dpf, embryos injected with 100 pg tsg1 RNA show a range of dorsalized phenotypes. (F) Uninjected sibling. (G) Class 1 dorsalization, arrowhead indicates gap in ventral tail fin. (H) Class 2 dorsalization, inset shows duplication in fin fold tissue seen in 50% of class 1 and class 2 embryos. (I) Class 3 phenotype.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT standard conditions 5-9 somites presumptive rhombomere 3 ISH
5-9 somites presumptive rhombomere 5 ISH
myod1 WT standard conditions 5-9 somites mesoderm ISH
pax2a WT standard conditions 5-9 somites midbrain hindbrain boundary neural rod ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development