FIGURE SUMMARY
Title

Inactivation of zebrafish mrf4 leads to myofibril misalignment and motor axon growth disorganization

Authors
Wang, Y.H., Li, C.K., Lee, G.H., Tsay, H.J., Tsai, H.J., and Chen, Y.H.
Source
Full text @ Dev. Dyn.
Fig. 2

Morphology of the wild-type (WT) embryos (A), mrf4_ (tv1:tv2)-morphants (B), mrf4_tv1-morphants (C), and mrf4_tv2-morphants (D), developed at 36 hpf were observed with a DMR microscope.

Fig. 3

F59 monoclonal antibody-staining of the 36-hpf of WT zebrafish embryos (A; cross section at E), mrf4_(tv1:tv2)-morphants (B,F), mrf4_tv1-morphants (C,G), and mrf4_tv2-morphants (D,H). White dashed lines in A and C indicate the boundaries of chevron-shaped somites. Yellow dashed lines in B and D indicate the boundaries of U-shaped somites.

Fig. 4

Expression patterns of α-actin, tnnt1, and tnnt3b transcripts at 27 hpf in WT embryos (A,A′, E,E′, I,I′), mrf4_(tv1:tv2)-morphants (B,B′, F,F′, J,J′), mrf4_tv1-morphants (C,C′, G,G′, K,K′), and mrf4_tv2-morphants (D,D′, H,H′, L,L′).

Fig. 5

Znp1 monoclonal antibody-staining (A, D, G, J) and α-bungarotoxin labeling (B, E, H, K) of the 27 hpf of WT zebrafish embryos (A, B, C), mrf4_(tv1:tv2)-morphants (D-F), mrf4_tv1-morphants (G-I), and mrf4_tv2-morphants (J-L). Merged pictures: (C) A and B; (F) D and E; (I) G and H; (L) J and K. C, F, I, L: The yellow signals indicate that the Znp1-positive cells are also α-bungarotoxin positive.

Fig. 6

Zn5 monoclonal antibody-labeling of the 54 hpf of WT zebrafish embryos (A, higher magnification at A′), mrf4_(tv1:tv2)-morphants (B, B′), mrf4_tv1-morphants (C, C′), and mrf4_tv2-morphants (D, D′).

Fig. S1

Expression patterns of mrf4_tv1 (A-D) and mrf4_tv2 (E-H) during early embryonic stages. A: At 18 hours postfertilization (hpf), lateral view. B: Head region of 24-hpf embryo, dorsal view. C: Trunk region of 24-hpf embryo, lateral view. D: Cross-section along the plane indicated by line D in C. E: At 18-hpf, lateral view. F: Trunk region of 24-hpf embryo, lateral view. G: At 48-hpf, dorsal and lateral view (H).

EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Long-pec
Fig. S2

RT-PCR analysis to test the targeting efficiency for each MO, using total RNA extracted from different injection groups. The primers P1 and P2 were used for detecting splicing variants of embryos derived from WT and mrf4_(tv1:tv2)-morphants (A), mrf4_tv1- and mrf4_tv2-morphants (B).

Fig. S3

Injection of Mrf4-MO does not affect the expression of myf5. A: WT embryo at 12-hpf, dorsal view. B: mrf4_(tv1:tv2)-morphants at 12-hpf, dorsal view.

EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 5-9 somites
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Dyn.
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