|
Fig. 6 RT-qPCR validation of changes in mRNA expression levels of immune genes normalized to mRNA expression levels in wild-type (WT). (A) Significant differences in gene expression between the nos2?/? mutant group and the wild-type (WT) group. (B) The horizontal axis is the gene name, the vertical axis is the microorganism; the asterisk indicates that the p-value is less than 0.05, which is represented by *; the color indicates the correlation, red indicates the positive correlation, and blue indicates the positive correlation. (C) mRNA expression levels of upregulated genes. (D) mRNA expression levels of downregulated genes.** Data represent three independent experiments. Note: Asterisks (*) denote statistical significance; ?ns? indicates no statistical significance; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.