Knockdown of wbp11 causes fin developmental defects in zebrafish. (A,B) Whole-mount in situ hybridization of zebrafish larvae at 48 hpf using the tbx5a probe to assess fin development. Scale bar: 250 μm (applies to (A,B)). (C–F) Bright field microscopy observation of fin morphology in zebrafish larvae at 96 hpf. Scale bar: 200 μm (applies to (C–F)). (G) Quantification of dorsal fin area (n = 10; **: p < 0.01). (H) Quantification of caudal fin area (n = 10; ***p < 0.001). (I) Quantification of anal fin area (n = 10; ***: p < 0.001). (J) Quantification of pelvic fin area (n = 10; ***p < 0.001). (K) Heatmap analysis of fin-related differentially expressed genes (DEGs). Blue indicates downregulated genes, and red indicates upregulated genes. (L) FPKM values of representative fin-related genes prdm1, hoxc13a, and hoxb13a (n = 3; biologically independent experiments, each performed with 50 pooled embryos per group. Data are presented as mean ± SEM. ***p < 0.001). (M) qPCR analysis of transcript levels of representative fin related genes prdm1a, hoxc13a, and hoxb13a (n = 3; biologically independent experiments, each performed with 50 pooled embryos per group. Data are presented as mean ± SEM. ***p < 0.001).
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