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Validation of wbp11 knockdown efficiency using morpholino in zebrafish. (A) Schematic diagram illustrating the targeting site of wbp11-MO. (B) Western blot analysis of wbp11 protein expression in zebrafish embryos at 72 hpf and 96 hpf to assess wbp11-MO knockdown efficiency. (C) Representative bright-field image of a zebrafish larva at 72 hpf following injection with Control-MO. (D–F) Representative bright-field images of zebrafish larvae at 72 hpf following injection with wbp11-MO, showing mild (D), moderate (E), and severe (F) malformation phenotypes. (G) Comparative bright-field observation of early embryonic development (2.5–24 hpf) in zebrafish embryos injected at the one-cell stage with Control-MO or wbp11-MO. Black scale bar: 250 μm; red scale bar: 100 μm (applies to C–F).
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