p53 activation contributes to excessive apoptosis in tubgcp6 mutants.
A Heatmap of sample-to-sample distance of tubgcp6 mutants and their sibling embryos. The color scale was shown on the right. The deeper the blue block was, the less different the samples were. B KEGG pathway analysis of RNA-seq data showed enrichment of the cell cycle and p53 signaling pathway in tubgcp6 mutants. C Heatmap demonstrated the upregulated genes in the p53 signaling pathway genes in tubgcp6 mutants. D RT-qPCR validated the significant up-regulation of p53-related genes in tubgcp6 mutants (mean ± SD; n = 3 independent experiments; Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001). E Double staining of cd41:eGFP (green) and TUNEL (red) in the CHT region of embryos at 3 dpf following injection of NC MO or p53 MO. Yellow arrows indicated TUNEL-positive cd41:eGFPlow HSPCs. Scale bars: 100 μm. F Quantification of percentage of co-stained TUNEL-positive cd41:eGFPlow HSPCs in the CHT region in each group of embryos (mean ± SD; n > 10; two-way ANOVA, ns p > 0.05, *p < 0.05, ***p < 0.001). Data from three independent experiments were pooled for analysis.
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