FIGURE

Figure S2.

ID

ZDB-FIG-260530-15

Publication

Pirzada et al., 2026 - Loss of U11/U12 spliceosome gene ZCRB1 leads to aberrant ciliogenesis and WNT signaling

Other Figures

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Figure S2.

Screening the CRISPR-Cas9-edited ZCRB1-heterozygous HEK293 cells.
(A, B) Western blot for ZCRB1 showing 16 selected clones with HSP70 as a loading control and GFP (donor) as a positive control for CRISPR-Cas9-edited cells, quantified in (B). (C)ZCRB1 mRNA levels in heterozygous knockout clones 33, 54, 63, and PL3C7 versus WT HEK293 Flp-In cell mRNA levels as quantified by qRT-PCR. mRNA levels relative to the housekeeping gene RPL27A for each clone are shown as a percentage of WT expression. Error bars in C represent the mean ± s.d. P-values are shown and were measured by an ordinary one-way ANOVA with Dunnett’s multiple comparisons test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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