Figure S2.
- ID
- ZDB-IMAGE-260530-22
- Publication
- Pirzada et al., 2026 - Loss of U11/U12 spliceosome gene ZCRB1 leads to aberrant ciliogenesis and WNT signaling
- All Figures
- Figures for Pirzada et al., 2026
|
Figure S2.
Screening the CRISPR-Cas9-edited ZCRB1-heterozygous HEK293 cells.
(A, B) Western blot for ZCRB1 showing 16 selected clones with HSP70 as a loading control and GFP (donor) as a positive control for CRISPR-Cas9-edited cells, quantified in (B). (C)ZCRB1 mRNA levels in heterozygous knockout clones 33, 54, 63, and PL3C7 versus WT HEK293 Flp-In cell mRNA levels as quantified by qRT-PCR. mRNA levels relative to the housekeeping gene RPL27A for each clone are shown as a percentage of WT expression. Error bars in C represent the mean ± s.d. P-values are shown and were measured by an ordinary one-way ANOVA with Dunnett’s multiple comparisons test.