Figure 4

Intracellular Ca²⁺ contributes to the response to hypoxia in neuroepithelial cells (NECs)

A, Ca²⁺ imaging trace from a GCaMP‐containing NEC, where the response to hypoxia was reversibly reduced with the addition of 50 µm dantrolene, an inhibitor of intracellular Ca²⁺ release. B, Ca²⁺ imaging trace from a GCaMP‐containing NEC demonstrating the combined contributions of intracellular and extracellular Ca²⁺. The response to hypoxia was further reduced with the addition of 50 µm dantrolene in Ca²⁺‐free extracellular solution. C, summary data treated as in (A) showing reduction in the mean ± SD F/F₀ (Kruskal–Wallis test, P = 0.007, n = 6 cells). The response to hypoxia fully recovered (Kruskal–Wallis test, P > 0.999, n = 6 cells). D, summary data treated as in (B) showing a reduction in the mean ± SD F/F₀ (Kruskal–Wallis test, P < 0.008, n = 5 cells). The response to hypoxia fully recovered (Kruskal–Wallis test, P > 0.999, n = 5 cells). E, summary comparing all Ca²⁺ blocking treatments. The combination of dantrolene with Ca²⁺‐free extracellular solution resulted in an additive effect compared to dantrolene alone. [Colour figure can be viewed at wileyonlinelibrary.com]