Figure 5

The effects of D₂R activity on the neuroepithelial cell (NEC) response to hypoxia

Showing the effects of dopamine (A and B), quinpirole (C and D) and domperidone (E and F). A, Ca²⁺ imaging trace from a GCaMP‐containing NEC where the response to hypoxia was reversibly reduced with the addition of 50 µm dopamine (DA). C, Ca²⁺ imaging trace from a GCaMP‐containing NEC where the response to hypoxia was reversibly reduced with the addition of 50 µm quinpirole, a specific dopamine D₂R agonist. E, Ca²⁺ imaging trace from a GCaMP‐containing NEC where the response to hypoxia was enhanced with the addition of 100 µm domperidone, a specific dopamine D₂R antagonist. B, D and F, summary data showing the mean ± SD. F/F₀ corresponding to experiments in (A), (C) and (E). Addition of 50 µm dopamine significantly reduced the Ca²⁺ response to hypoxia (Kruskal–Wallis test, P = 0.012, n = 5 cells) (B), as well as 50 µm quinpirole (Kruskal–Wallis test, P = 0.007, n = 6 cells) (D), wheras domperidone increased the mean ± SD F/F₀ (Kruskal–Wallis test, P = 0.035, n = 6 cells) (F). The response to hypoxia fully recovered following all treatments (Kruskal–Wallis test, P > 0.999, n = 5–6 cells). [Colour figure can be viewed at wileyonlinelibrary.com]