FIGURE

Fig. 3

ID
ZDB-FIG-250305-21
Publication
Ma et al., 2025 - Establishment and application of a zebrafish model of Werner syndrome identifies sapanisertib as a potential antiaging drug
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Fig. 3

Drug screening identified that sapanisertib alleviated aging phenotypes in wrnKO mutants. (A) Schematic illustration of antiaging drug screening on the zebrafish wrnKO mutant. (B) Representative image of SA-?-Gal staining and quantification of the intensity of SA-?-Gal staining in the intestine at 8 dpf (n = 12 to 15 for each group). Arrows indicate the intestine region. (C) qPCR analysis of age-related gene expression (p21 and p53) in intestinal samples at 8 dpf (n = 40 for each group). (D) Western blot images of p53 in WT, wrnKO+DMSO, and wrnKO+Sapa groups in intestinal samples at 8 dpf (n = 40 for each group). (E) The percent of survival of the WT (n = 19), wrnKO+DMSO (n = 24), and wrnKO +Sapa (n = 18) groups from 0 dpf to 15 dpf is presented. (F) Representative swimming tracks (Left) and quantification of total distance traveled and swimming speed (Right) among WT, wrnKO + DMSO, and wrnKO +Sapa groups at 15 dpf (n = 10 for each group). The yellow circle represents the recording field, and the red line represents the swimming track. (G) ORO staining and WISH of cetp at 8 dpf. Numbers indicate the proportion of larvae exhibiting the expression shown. (H) Single-optical section images showing the expression of ?H2AX (red), Dendra2 (green), and DAPI (blue) in the intestine of Tg(fabp2:Dendra2-NTR) at 8 dpf. Quantification of the percent of ?H2AX (n = 10 for each group). (Scale bar, 50 ?m.) Data are presented as mean ± SEM. Significance was determined by one-way ANOVA, and the survival rate was analyzed with the log-rank test. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Sapa, sapanisertib.

Expression Data
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage Range: Days 7-13 to Days 14-20

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA