Figure 2

Impaired convergent extension in dact1/2 compound mutants. (A) Inter-cross of compound heterozygotes yield embryos with different degrees of axis extension that correspond to the dact1 and dact2 genotypes. Representative lateral images of embryos at 12 hpf. The yellow line indicates body axis angle measured from the anterior point of the head, the center of the yolk, to the end of the tail. (B) Quantification of body axis angle. Numbers represent the difference in angle relative to the average wildtype embryo. Asterisk indicates genotypes with angles significantly different from wildtype. ANOVA p < 0.5 n = 3?21 embryos. Error bars: ± SEM. (C) Representative bud stage wildtype and dact1/2?/? mutant embryos stained for gsc (prechordal plate), pax2a (midbrain/hindbrain boundary), and krox20 (rhombomere 3). Asterisk indicates lack of krox20 expression in dact1/2?/? mutant. Scale bar = 200 ?m (D) Representative flat mounts of 1?2 ss wildtype and dact1/2 mutant embryos stained for zic1 (telencephalon), pax2a and tbx6 (ventrolateral mesoderm). (E) Representative flat mounts of 10 ss wildtype and dact1/2?/? mutant embryos stained for ctsl1b (hatching gland), zic1, pax2a, krox20, and myo1d (somites).