FIGURE

Figure 3.

ID

ZDB-FIG-241122-16

Publication

Carroll et al., 2024 - Genetic requirement of dact1/2 to regulate noncanonical Wnt signaling and calpain 8 during embryonic convergent extension and craniofacial morphogenesis

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Figure 3.

Midface development requires dact1 and dact2.

(A) Representative brightfield images of wildtype and dact1/2−/− compound mutants at 4 dpf. 100 individuals were analyzed from a dact1/2+/- double het cross. Lateral and ventral views show dact1/2−/− compound mutants have a hypoplastic midface, medially displaced eyes, and a displaced lower jaw. (B) Representative flat-mount images of Alcian blue stained ethmoid plate (EP) and visceral cartilage (VC) elements from 4 dpf wildtype and dact1/2−/− compound mutants. dact1/2−/− mutants have a rod-shaped EP with no distinct lateral and medial elements. No obvious differences were found in dact1/2 mutant VC. (C) Representative brightfield image of 4 dpf wildtype and dact1/2−/− mutant. Bar indicates vertebral spine length. Scale bar: 100 μm. (D) Representative images of Alcian blue stained dact1/2−/−, wnt11f2−/−, and wnt11f2−/−,dact1/2−/− compound mutants. Embryos resulted from a dact1+/-,dact2+/-,wnt11f2+/-in-cross. Lateral and ventral views show similar craniofacial phenotypes in each mutant. (E) Representative flat-mount images of Alcian blue stained EP show a similar phenotype between dact1/2−/−, wnt11f2−/−, and wnt11f2−/−,dact1/2−/− compound mutants. Scale bar: 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	dact1^{zf4284/zf4284}; dact2^{zf4285/zf4285} wnt11f2^tx226/tx226; dact1^{zf4284/zf4284}; dact2^{zf4285/zf4285}
Observed In:	ethmoid cartilage eye left side eye right side mandibular arch skeleton whole organism
Stage:	Day 4

Phenotype Detail

Acknowledgments

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